Test Catalog

Test Id : RTIC

Reticulocytes, Blood

Useful For
Suggests clinical disorders or settings where the test may be helpful

Assessing erythropoietic bone marrow activity in anemia and other hematologic conditions

Method Name
A short description of the method used to perform the test

Flow Cytometry

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Reticulocytes, B

Specimen Type
Describes the specimen type validated for testing

Whole Blood EDTA

Necessary Information

Specimen must arrive within 48 hours of draw.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1.5 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Other Clotted

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated (preferred) 48 hours
Ambient 24 hours

Useful For
Suggests clinical disorders or settings where the test may be helpful

Assessing erythropoietic bone marrow activity in anemia and other hematologic conditions

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Reticulocytes are immature erythrocytes (RBC) that have been released into the peripheral blood from the bone marrow after extrusion of their nucleus. The reticulocyte contains residual polyribosomes used in the formation of hemoglobin in the developing erythrocyte.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

% RETICULOCYTES

1-3 days: 3.47-5.40%

4 days-4 weeks: 1.06-2.37%

5 weeks-7 weeks: 2.12-3.47%

8 weeks-5 months: 1.55-2.70%

6 months-23 months: 0.99-1.82%

24 months-5 years: 0.82-1.45%

6-11 years: 0.98-1.94%

12-17 years: 0.90-1.49%

Adults: 0.60-2.71%

 

ABSOLUTE RETICULOCYTES

1-3 days: 147.5-216.4 x 10(9)/L

4 days-4 weeks: 51.3-110.4 x 10(9)/L

5 weeks-7 weeks: 51.8-77.9 x 10(9)/L

8 weeks-5 months: 48.2-88.2 x 10(9)/L

6 months-23 months: 43.5-111.1 x 10(9)/L

24 months-5 years: 36.4-68.0 x 10(9)/L

6-11 years: 42.4-70.2 x 10(9)/L

12-17 years: 41.6-65.1 x 10(9)/L

Adults: 30.4-110.9 x 10(9)/L

Interpretation
Provides information to assist in interpretation of the test results

The reticulocyte count is a measure of the number of RBCs delivered by the bone marrow. It is elevated with active erythropoiesis such as regeneration, and is decreased in hypoplastic or deficiency conditions such as vitamin B12 deficiency.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Reticulocyte counts must be carefully correlated with other clinical and laboratory findings.

 

Clotted specimens yield unreliable results and are unacceptable for analysis.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Adeli K, Raizman J, Chen Y, et al: Complex biological profile of hematologic markers across pediatric, adult, and geriatric ages: establishment of robust pediatric and adult reference intervals on the basis of the Canadian Health Measures Survey. Clin Chem 2015 Aug;61(8):1075-1086

2. CLSI. Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline, Third Edition. CLSI document EP28-A3c. Wayne, PA, Clinical and Laboratory Standards Institute, 2008

3. Soldin J, Brugnara C, Wong EC: Pediatric Reference Intervals. Fifth edition. AACC Press. Washington DC 2005. ISBN 1-594250-32-4

4. Clinical Hematology: Principles, procedures, correlations. Second edition. Edited by CA Lotspeich-Steininger, EA Stiene-Martin, JA Koepke. Philadelphia, Lippincott-Raven, 1998, pp 114-117

Method Description
Describes how the test is performed and provides a method-specific reference

The Sysmex XN 9000 reticulocyte analyzer is a flow cytometer that uses an argon laser as the light source. Whole blood specimens are stained with polymethine fluorescent dye and passed through the laser beam in a sheath flow cell. Each blood cell causes a forward light scatter that depends on cell size. This measurement permits a separation of the various blood cell types so that only erythrocytes are included in the cell count. Erythrocytes containing the protein reticulin (ie, reticulocytes) are stained by the dye in the laser beam. The right-angle fluorescence is measured to count these stained cells. The instrument counts 30,000 erythrocytes in each specimen. Because a known aliquot is counted, an actual RBC per unit volume is performed and an absolute reticulocyte count is obtained.(Instruction manual: Automated Hematology Analyzer XN series [XN-9000], North American edition, Code No. CJ410539. November 2015)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 day

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

85045

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports