Work-up of congenital hemolytic anemias
Only orderable as part of a profile or as a reflex. For more information see:
-HAEV1 / Hemolytic Anemia Evaluation
-HBEL1 / Hemoglobin Electrophoresis Evaluation, Blood
-THEV1 / Thalassemia and Hemoglobinopathy Evaluation
-REVE1 / Erythrocytosis Evaluation
-MEV1 / Methemoglobinemia Evaluation
Isopropanol and Heat Stability
Whole Blood EDTA
Only orderable as part of a profile or as a reflex. For more information see:
-HAEV1 / Hemolytic Anemia Evaluation
-HBEL1 / Hemoglobin Electrophoresis Evaluation, Blood
-THEV1 / Thalassemia and Hemoglobinopathy Evaluation
-REVE1 / Erythrocytosis Evaluation
-MEV1 / Methemoglobinemia Evaluation
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
1 mL
Gross hemolysis | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Refrigerated (preferred) | 7 days |
Work-up of congenital hemolytic anemias
Unstable hemoglobin disease is rare and may be caused by any
The stained peripheral blood smear shows anisocytosis, poikilocytosis,
Only orderable as part of a profile or as a reflex. For more information see:
-HAEV1 / Hemolytic Anemia Evaluation
-HBEL1 / Hemoglobin Electrophoresis Evaluation, Blood
-THEV1 / Thalassemia and Hemoglobinopathy Evaluation
-REVE1 / Erythrocytosis Evaluation
-MEV1 / Methemoglobinemia Evaluation
Normal (reported as normal [stable] or abnormal [unstable])
An abnormal or unstable result is indicative of a hemoglobin variant present. Other confirmatory tests should be performed to identify the hemoglobinopathy (HBEL1 / Hemoglobin Electrophoresis Cascade, Blood).
False-positive results will be obtained in blood specimens containing greater than 5% fetal hemoglobin or in specimens greater than 1 week old.
1. Hoyer JD, Hoffman DR: The thalassemia and hemoglobinopathy syndromes. In: McClatchey KD, Amin HM, Curry JL, eds. Clinical Laboratory Medicine. 2nd ed. Lippincott Williams and Wilkins; 2002:866-895
2. Benz EJ, Ebert BL: Hemoglobin variants associated with hemolytic anemia, altered oxygen affinity, and methemoglobinemias. In: Hoffman R, Benz EJ, Silberstein LE, et al: eds. Hematology: Basic Principles and Practice. 7th ed. Elsevier; 2018:608-615
Unstable hemoglobins precipitate in dilute solutions of isopropanol. Washed erythrocytes are hemolyzed and cleared by centrifugation. Isopropanol is added. The hemolysate is incubated at 37 degrees C for 20 minutes and examined for turbidity. There is no turbidity with normal hemoglobins.(Schmidt RM: Laboratory diagnosis of hemoglobinopathies. In: Bick RL ed. Hematology Clinical and Laboratory Practice. Mosby-Year Book Inc; 1993, pp 327-389; Greene DN, Vaughn CP, Crews BO, Agarwal AM: Advances in the detection of hemoglobinopathies. Clinica Chimica Acta. 2015;439:50-57)
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
83068
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
UNHB | Hb Stability, B | 4639-1 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
9095 | Hb Stability, B | 4639-1 |