Test Catalog

Test Id : ENAE

Antibody to Extractable Nuclear Antigen Evaluation, Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

Evaluating patients with signs and symptoms of a connective tissue disease in whom the test for antinuclear antibodies is positive

 

Testing is not useful in patients without demonstrable antinuclear antibodies.

Profile Information
A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test Id Reporting Name Available Separately Always Performed
SSA SS-A/Ro Ab, IgG, S Yes Yes
SSB SS-B/La Ab, IgG, S Yes Yes
SM Sm Ab, IgG, S Yes Yes
RNP RNP Ab, IgG, S Yes Yes
SCL70 Scl 70 Ab, IgG, S Yes Yes
JO1 Jo 1 Ab, IgG, S Yes Yes

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Connective Tissue Disease Cascade (CTDC) in Special Instructions.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Multiplex Flow Immunoassay

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Ab to Extractable Nuclear Ag Eval,S

Aliases
Lists additional common names for a test, as an aid in searching

Anti-ENA (Antibodies to Extractable Nuclear Antigens)

Anti-La

Anti-Ribonuclear Protein

Anti-RNP (Anti-Ribonucleoprotein)

Anti-Ro

Anti-Sjogren's Syndrome

Anti-Sm (Smith)

Anti-Smith (SM) Antigen

Anti-SSA (Anti-Sjogren's Syndrome A)

Anti-SSB (Anti-Sjogren's Syndrome B)

Anti-U(1)RNP (Anti-Ribonucleoprotein)

Antibodies to Extractable Nuclear Antigens

Autoantibodies to Jo 1 Antigen

Autoantibodies to Sm (Smith)

Autoantibodies to SSA (Ro)

Autoantibodies to SSB (La)

Autoantibodies to U(1)RNP

Connective Tissue Disease Autoantibody Panel (2 tests)

DNA Topoisomerase 1 Antibodies

ENA (Extractable Nuclear Antigens), Anti

ENA/ADNA

Extractable Nuclear Antigens, Anti

Histidyl-tRNA Synthetase Antibodies

Immunology Profile (ENA)

Jo 1 Antibodies

LA (Anti-La)

La (Anti-SSB)

Polymyositis Antibodies

Ribonucleoprotein (RNP) Autoantibodies

RNP (Anti-RNP)

Ro (Anti-SSA)

Scl 70 Antibodies

Scleroderma Antibodies (? FOR SPECIFIC TEST)

Sjogren's Syndrome

Sm (Anti-Sm) (Smith)

Sm (Smith) Autoantibodies

Smith Antigen, Anti

SmRNP Autoantibodies

SSA (Anti-SSA)

SSB (Anti-SSB)

Topoisomerase 1 Antibodies

U(1)RNP (Ribonucleoprotein) Autoantibodies

Autoantibodies to Scl 70 Antigen

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Connective Tissue Disease Cascade (CTDC) in Special Instructions.

Specimen Type
Describes the specimen type validated for testing

Serum

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 0.5 mL

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.35 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
Frozen 21 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Evaluating patients with signs and symptoms of a connective tissue disease in whom the test for antinuclear antibodies is positive

 

Testing is not useful in patients without demonstrable antinuclear antibodies.

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Connective Tissue Disease Cascade (CTDC) in Special Instructions.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

SSA, SSB, SM, and RNP are extractable nuclear antigens (ENA) that occur in patients with several different connective tissue diseases. Scl 70 (topoisomerase 1) is a 100-kD nuclear and nucleolar enzyme. Scl 70 antibodies are considered to be specific for scleroderma (systemic sclerosis) and are found in up to 60% of patients with this connective tissue disease. Scl 70 antibodies are more common in patients with extensive cutaneous involvement and interstitial pulmonary fibrosis, and are considered a poor prognostic sign.(1) JO1 is a member of the amino acyl-tRNA synthetase family of enzymes found in all nucleated cells and a marker for the disease polymyositis.

 

For more information, see individual unit codes.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

SS-A/Ro ANTIBODIES, IgG

<1.0 U (negative)

> or =1.0 U (positive)

Reference values apply to all ages.

 

SS-B/La ANTIBODIES, IgG

<1.0 U (negative)

> or =1.0 U (positive)

Reference values apply to all ages.

 

Sm ANTIBODIES, IgG

<1.0 U (negative)

> or =1.0 U (positive)

Reference values apply to all ages.

 

RNP ANTIBODIES, IgG

<1.0 U (negative)

> or =1.0 U (positive)

Reference values apply to all ages.

 

Scl 70 ANTIBODIES, IgG

<1.0 U (negative)

> or =1.0 U (positive)

Reference values apply to all ages.

 

Jo 1 ANTIBODIES, IgG

<1.0 U (negative)

> or =1.0 U (positive)

Reference values apply to all ages.

Interpretation
Provides information to assist in interpretation of the test results

A positive result is consistent with a connective tissue disease. 

 

For more information, see individual unit codes.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

No significant cautionary statements

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Homburger H, Larsen S: Detection of specific antibodies. In Clinical Immunology: Principles and Practice. First edition. Edited by R Rich, T Fleisher, B Schwartz, et al. St. Louis, Mosby-Year Book, Inc, 1996, pp 2096-2109

2. Targoff I: Autoantibodies in polymyositis. Rheum Dis Clin North Am 1992;18:455

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

See individual unit codes.

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Saturday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 2 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

86235 x 6

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
ENAE Ab to Extractable Nuclear Ag Eval,S 90228-8
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
JO1 Jo 1 Ab, IgG, S 33571-1
RNP RNP Ab, IgG, S 29958-6
SCL70 Scl 70 Ab, IgG, S 47322-3
SM Sm Ab, IgG, S 18323-6
SSA SS-A/Ro Ab, IgG, S 33610-7
SSB SS-B/La Ab, IgG, S 33613-1

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports