Test Catalog

Test Id : HDCH

Cholesterol, High-Density Lipoprotein (HDL), Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

Cardiovascular risk assessment

Method Name
A short description of the method used to perform the test

Enzymatic Colorimetric

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Cholesterol, HDL, S

Aliases
Lists additional common names for a test, as an aid in searching

Cholesterol, HDL (High Density Lipoprotein)

HDL Direct

High Density Lipoprotein, Cholesterol

Specimen Type
Describes the specimen type validated for testing

Serum

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Patient Preparation: Preferred but not required

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tube must be centrifuged within 2 hours of draw time.

2. Red-top tube must be centrifuged and the serum aliquoted within 2 hours of draw time.

Forms

If not ordering electronically, complete, print, and send a Cardiovascular Test Request (T724) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.25 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
Frozen 30 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Cardiovascular risk assessment

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

High-density lipoprotein (HDL) is the smallest of the lipoprotein particles and comprises a complex family of lipoprotein particles that exist in a constant state of dynamic flux as the particles interact with other HDL particles and with low-density lipoprotein particles and very-low-density lipoprotein particles. HDL has the largest proportion of protein relative to lipid compared to other lipoproteins (>50% protein).

 

Total cholesterol levels have long been known to be related to coronary heart disease (CHD). HDL cholesterol is also an important tool used to assess an individual's risk of developing CHD since a strong negative relationship between HDL cholesterol concentration and the incidence of CHD has been reported.

 

In some individuals, exercise increases the HDL cholesterol level; those with more physical activity have higher HDL cholesterol values.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

The National Lipid Association and the National Cholesterol Education Program (NCEP) have set the following guidelines for lipids (total cholesterol, triglycerides, high-density lipoprotein [HDL] cholesterol, low-density lipoprotein [LDL] cholesterol, and non-HDL cholesterol) in adults 18 years old and older:

HDL CHOLESTEROL

Males

> or =40 mg/dL

Females

> or =50 mg/dL

 

The Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents has set the following guidelines for lipids (total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol, and non-HDL cholesterol) in children 2-17 years old:

HDL CHOLESTEROL

Low HDL: <40 mg/dL

Borderline low: 40-45 mg/dL

Acceptable: >45 mg/dL

Interpretation
Provides information to assist in interpretation of the test results

Low high-density lipoprotein (HDL) cholesterol levels correlate with increased risk for coronary heart disease (CHD). Values greater than or equal to 80 to 100 mg/dL may indicate metabolic response to certain medications such as hormone replacement therapy, chronic liver disease, or some form of chronic intoxication, such as with alcohol, heavy metals, or industrial chemicals including pesticides.

 

HDL values of 5 mg/dL or less occur in Tangier disease, in association with cholestatic liver disease, and in association with diminished hepatocyte function.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Fasting is not necessary but is preferable. However, if the high-density lipoprotein (HDL) cholesterol data is used to calculate the low-density lipoprotein (LDL) cholesterol, fasting is required since triglycerides concentration is used in the calculation.

 

Result can be falsely decreased in patients with elevated levels of N-acetyl-p-benzoquinone imine (NAPQI, a metabolite of acetaminophen), N-acetylcysteine (NAC), and metamizole.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Burtis CA, Ashwood ER, eds: Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Elsevier Saunders; 2012

2. Rifai N, Warnick GR: Laboratory Measurement of Lipids, Lipoproteins, and Apolipoproteins. AACC Press; 1994

3. Jacobson TA, Ito MK, Maki KC, et al: National Lipid Association recommendations for patient-centered management of dyslipidemia: Part 1 - executive summary. J Clin Lipidol. 2014 Sep-Oct;8(5):473-488. doi: 10.1016/j.jacl.2014.07.007

4. Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents; National Heart, Lung, and Blood Institute. Expert panel on integrated guidelines for cardiovascular health and risk reduction in children and adolescents: summary report. Pediatrics. 2011;128;S213. doi: 10.1542/peds.2009-2107C

Method Description
Describes how the test is performed and provides a method-specific reference

Non high-density lipoprotein (HDL) lipoproteins such as low-density lipoprotein, very low-density lipoprotein, and chylomicrons are combined with polyanions and a detergent forming a water-soluble complex. In this complex, the enzymatic reaction of cholesterol esterase (CHER) and cholesterol oxidase (CHOD) towards non-HDL lipoproteins is blocked. Finally, only HDL-particles can react with CHER and CHOD. The concentration of HDL-cholesterol is determined enzymatically by CHER and CHOD. Cholesterol esters are broken down quantitatively into free cholesterol and fatty acids by CHER. In the presence of peroxidase, the hydrogen peroxide generated reacts with 4-amino-antipyrine and N-ethyl-N-(3-methylphenyl)-N’-succinylethylenediamine) to form a dye. The color intensity of this dye is directly proportional to the cholesterol concentration and is measured photometrically.(Package insert: HDL-Cholesterol Gen4. Roche Diagnostics; V 2.0, 08/2018)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 day

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

83718

LOINC® Information

Test Id Test Order Name Order LOINC Value
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
HDCH Cholesterol, HDL, S 2085-9
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
HDCH Cholesterol, HDL, S 2085-9

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Create a PDF

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports