Test Catalog

Test Id : LKM

Liver/Kidney Microsome Type 1 Antibodies, Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

Evaluation of patients with liver disease of unknown etiology


Evaluation of patients with suspected autoimmune hepatitis

Method Name
A short description of the method used to perform the test

Enzyme-Linked Immunosorbent Assay (ELISA)

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Liver/Kidney Microsome Type 1 Ab, S

Lists additional common names for a test, as an aid in searching

Antibodies to Liver\Kidney Microsome Type 1, Serum

LKM1 (liver/kidney microsome type 1) antibodies

Microsomal (Liver/Kidney) Type 1 Antibodies

aLKM 1

Specimen Type
Describes the specimen type validated for testing


Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.4 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
Frozen 21 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Evaluation of patients with liver disease of unknown etiology


Evaluation of patients with suspected autoimmune hepatitis

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Autoimmune liver disease (eg, autoimmune hepatitis and primary biliary cirrhosis) is characterized by the presence of autoantibodies including smooth muscle antibodies (SMA), antimitochondrial antibodies (AMA), and anti-liver/kidney microsomal antibodies type 1 (anti-LKM-1).(1) Subtypes of autoimmune hepatitis (AIH) are based on autoantibody reactivity patterns.


Anti-LKM-1 antibodies serve as a serologic marker for AIH type 2 and typically occur in the absence of SMA and antinuclear antibodies. These antibodies react with a short linear sequence of the recombinant antigen cytochrome monooxygenase P450 2D6.(2) Patients with AIH type 2 more often tend to be young, female, and have severe disease that responds well to immunosuppressive therapy.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

< or =20.0 Units (negative)

20.1-24.9 Units (equivocal)

> or =25.0 Units (positive)

Reference values apply to all ages.

Provides information to assist in interpretation of the test results

Seropositivity for anti-liver/kidney microsomal antibodies type 1 (anti-LKM-1) antibodies is consistent with a diagnosis of autoimmune hepatitis (AIH) type 2.

Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Serologic tests for autoantibodies, including anti-liver/kidney microsomal antibodies type 1 (anti-LKM-1), should not be relied upon exclusively to determine the etiology or prognosis of patients with liver disease.


Anti-LKM-1 antibodies may occur in some patients with chronic hepatitis caused by hepatitis C virus infection. Although the epitopes recognized by anti-LKM-1 antibodies in hepatitis C virus infection are different than in patients with autoimmune hepatitis (AIH) type 2, physicians must use caution in interpreting the results of tests for anti-LKM-1 antibodies in such patients.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Clinical Immunology Principles and Practice. Third edition. Edited by RR Rich, TA Fliesher, WT Shearer, et al: Philadelphia, PA, Mosby Elsevier, 2008

2. Czaja AJ, Homburger HA: Autoantibodies in liver disease. Gastroenterology. January 2001;120(1):239-249

Method Description
Describes how the test is performed and provides a method-specific reference

Enzyme-linked immunosorbent assay (ELISA) for the semi-quantitative detection of liver/kidney microsomal antibodies type 1 (LKM-1) antibodies in human serum.(Package insert: INOVA Diagnostics, Inc. San Diego, Revision 1, March 2000)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday, Wednesday, Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 4 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test


Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.


Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports