Test Catalog

Test Id : FIFLC

Immunofixation with Free Light Chains, Quantitative, Urine

Method Name
A short description of the method used to perform the test

Qualitative Immunofixation Electrophoresis/Quantitative Immunoturbidimetry

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Immunofix Free Light Chains, U

Lists additional common names for a test, as an aid in searching

Free Urinary Kappa Light Chains

Free Urinary Kappa/Lambda Ratio

Free Urinary Lambda Light Chain


IFE urine

Immunofixation Electrophoresis, urine

Monoclonal protein detection and quantitation, urine


Urinary Light Chains

Specimen Type
Describes the specimen type validated for testing



Question ID Description Answers
Z5897 Hours Collected
Z5898 Total Volume

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Specimen Type: Urine

Submission Container/Tube: Two 4-mL plastic aliquot tube(s)

Specimen Volume: 8 mL (Two 4-mL plastic aliquot tube(s))

Collection Instructions:

1. Collect urine for 24 hours (NO preservative).

2. Refrigerate specimen during the 24-hour collection.

3. Send specimen refrigerate in Two 4-mL plastic aliquot tube(s)

4. Collection volume and duration are required

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

4 mL (2 vials 2 mL each)

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Thawing** Cold OK; Warm reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 21 days
Frozen 180 days

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Total Protein

Less than 150 mg/d

Free Urinary Kappa Light Chains

0.00-32.90 mg/L

Free Urinary Kappa Excretion/Day

By report

Free Urinary Lambda Light Chain

0.00-3.79 mg/L

Free Urinary Lambda Excretion/Day

By report

IFE Interpretation

By report

Provides information to assist in interpretation of the test results

Results of urine free light chain testing can be used to monitor disease progression or response to therapy in patients for whom urine electrophoresis is unable to provide reliable Bence Jones Protein quantification. The results of urine kappa and lambda free light chain quantitative values may be misleading in specimens with high levels of urinary polyclonal free light chains, and absent Bence Jones protein by immunofixation; therefore correlation with urine immunofixation is required to identify inconsistent results. Total urinary protein is determined turbidmetrically by adding the albumin and kappa and/or lambda light chains. This value may not agree with the total protein as determined by chemical methods, which characteristically underestimates urinary light chains.

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 9 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

ARUP Laboratories

Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.



83520 x 2

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
FIFLC Immunofix Free Light Chains, U Not Provided
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
Z5897 Hours Collected 30211-7
Z5898 Total Volume 19153-6
Z5899 Total Protein 2889-4
Z5901 Free Urinary Kappa Light Chains 38176-4
Z5912 Free Urinary Kappa Excretion/Day 38177-2
Z5913 Free Urinary Lambda Light Chain 38178-0
Z5914 Free Urinary Lambda Excretion/Day 38169-9
Z5900 IFE Interpretation 49277-7

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports