Undetermined metabolic bone disease in wet tissue specimens
Renal osteodystrophy
Osteomalacia
Osteoporosis
Paget disease
Assessing effects of therapy
Identification of some disorders of the hematopoietic system
Aluminum toxicity
Presence of iron in the bone
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
SS2PC | SpecStain, Grp II, other | No, (Bill Only) | No |
Hematoxylin and eosin-stained slides are sent to pathology for review. Iron staining or aluminum staining may be performed at an additional charge.
Histomorphometry
Al (Aluminum) Stain
Aluminum (Al) Stain
Histomorphometry, Bone
Toluidine Blue
Bone Histomorphometry (always order BHISC)
Hematoxylin and eosin-stained slides are sent to pathology for review. Iron staining or aluminum staining may be performed at an additional charge.
Varies
It is preferred that the specimen is shipped in 70% ethanol because it preserves the tetracycline label in the bone.
Bone Histomorphometry: Patient Information (T352) in Special Instructions must be completed and sent with the specimen. The laboratory requires this information in order to perform testing
Supplies:
-Metal Free Specimen Vial (T173)
-Bone Histomorphometry Specimen Preparation (T579)
Specimen Type: Bone
Preferred: Anterior iliac crest
Container/Tube: Metal-free container (T173)
Specimen Volume: Entire specimen
Collection Instructions:
1. Fix specimen in 70% ethanol.
2. Quantitation of bone turnover requires 2 time-spaced tetracycline labels.
3. The use of metal-free containers is required to avoid aluminum or iron contamination.
Additional Information:
1. Consultation with a Mayo Clinic Laboratories pathologist or endocrinologist/nephrologist is recommended for first-time users of this service. Written instructions are available upon request.
2. For more information, see Bone Histomorphometry Specimen Preparation (T579) in Special Instructions.
Bone Histomorphometry: Patient Information (T352) in Special Instructions
Bone Histomorphometry Specimen Preparation (T579) in Special Instructions
Bone | Fixatives other than 70% alcohol or decalcified bone |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Ambient (preferred) | ||
Refrigerated |
Undetermined metabolic bone disease in wet tissue specimens
Renal osteodystrophy
Osteomalacia
Osteoporosis
Paget disease
Assessing effects of therapy
Identification of some disorders of the hematopoietic system
Aluminum toxicity
Presence of iron in the bone
Hematoxylin and eosin-stained slides are sent to pathology for review. Iron staining or aluminum staining may be performed at an additional charge.
Bone histomorphometry is a very sophisticated procedure utilizing full-thickness bone biopsy.
Techniques such as 2-time interval labeling with tetracycline permit the direct measurement of the rate of bone formation. The information derived is useful in the diagnosis of metabolic bone diseases, including renal osteodystrophy, osteomalacia, and osteoporosis, and other disorders such as aluminum toxicity and iron abnormalities.
The laboratory will provide a quantitative and an interpretive report.
Computer-generated histomorphometric values are given for adequate specimens.
Normal histomorphometric values for iliac crest are provided (female only).
An interpretive report will be provided.
Mineralization or bone formation rates can be done only when tetracycline has been administered on a specific schedule prior to biopsy.
Biopsy site of preference is iliac crest.
Dairy products should not be ingested at the same time as the tetracycline because they interfere with tetracycline uptake.
Recker RR: Bone Histomorphometry: Techniques and Interpretation. Boca Raton, FL, CRC Press, 1983
Intact (nonfragmented) specimens of undecalcified bone are dehydrated in ethanol, embedded in methylmethacrylate, sectioned at 5-micron thickness, and individual sections are stained with Goldner Trichrome, toluidine blue, and hematoxylin and eosin. Special stains for acid phosphatase, aluminum, and iron are performed when indicated. Histomorphometry, by computerized technique, is then done on the stained sections using light microscopy and on unstained sections using fluorescence microscopy. Histomorphometric values and the histologic appearance of all sections are reviewed and interpreted by a clinician-histomorphometrist. A hematoxylin and eosin-stained section is reviewed for abnormalities by a pathologist. Telephone consultations with responsible physicians are carried out whenever possible.(Hodgson SF, Johnson KA, Muhs JM, et al: Outpatient percutaneous biopsy of the iliac crest: methods, morbidity, and patient acceptance. Mayo Clin Proc 1986;61:28-33)
Monday through Friday
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
88346
88307
88313
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
BHISC | Bone HistoMorph Gross Micro Exam | 60570-9 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
71158 | Interpretation | 59465-5 |
71159 | Bone Marrow Interpretation | 51628-6 |
71160 | Participated in the Interpretation | No LOINC Needed |
71161 | Report electronically signed by | 19139-5 |
71162 | Material Received | 22633-2 |
71786 | Case Number | 80398-1 |
601908 | Disclaimer | 62364-5 |