This test may not be used independently for monitoring treatment response or establishing remission.
For more information see Connective Tissue Disease Cascade.
Only orderable as reflex. For more information see ADNAR / DNA Double-Stranded (dsDNA) Antibodies with Reflex, IgG, Serum.
Indirect Immunofluorescence
Anti-DNA
Anti-ds (Anti-Double Stranded) DNA
ANTI-dsDNA ANTIBODY, IgG
Antibody to ds-DNA
Antibody to Native DNA (n-DNA)
Connective Tissue Disease Activity Assessment (2 tests)
Connective Tissue Disease Autoantibody Panel (2 tests)
DNA Double Stranded (ds-DNA) Antibody
DNA, Anti
Double Stranded DNA
ds-DNA Antibody
IgG Anti-DS DNA Antibody
IgG Autoantibodies to Double Stranded (ds)DNA
LUPUS
Native Anti-DNA
Native DNA
SLE (Systemic Lupus Erythematosus)
Systemic Lupus Erythematosus (SLE)
For more information see Connective Tissue Disease Cascade.
Serum
Only orderable as reflex. For more information see ADNAR / DNA Double-Stranded (dsDNA) Antibodies with Reflex, IgG, Serum.
0.2 mL
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 21 days | |
Frozen | 21 days |
This test may not be used independently for monitoring treatment response or establishing remission.
For more information see Connective Tissue Disease Cascade.
Double-stranded DNA (dsDNA) antibodies are systemic lupus erythematosus (SLE)-specific antibodies and are part of the immunology domain of the 2019 European League Against Rheumatism (EULAR)/American College of Rheumatology (ACR) classification criteria for SLE (1) as well as a previous guidance on SLE diagnosis.(2) The Crithidia luciliae indirect immunofluorescence test (CLIFT) is widely used as a confirmatory test following a positive dsDNA IgG result obtained by a solid-phase immunoassay due to its structural or analytical specificity.(3-5)
A minority of SLE patients may test negative using HEp-2 by IFA for nuclear antibodies.(9) Testing antibodies associated with the HEp-2 IFA cytoplasmic pattern such as ribosomal P IgG autoantibodies may be useful if features of neuropsychiatric disease are present.(9) Alternatively, patients may be tested for Smith, ribonuclear protein (RNP), sulfosalicylic acid (SSA)-52 and SSA-60 antibodies.(6,9)
Only orderable as reflex. For more information see ADNAR / DNA Double-Stranded (dsDNA) Antibodies with Reflex, IgG, Serum.
Negative
A positive result for double-stranded DNA (dsDNA) IgG antibodies in the appropriate clinical context is highly suggestive of systemic lupus erythematosus (SLE). The presence of dsDNA IgG antibodies detected using the Crithidia luciliae indirect immunofluorescence test is highly specific for SLE with moderate sensitivity. A negative result does not rule out a diagnosis of SLE.
IgG antibodies to double-stranded DNA (dsDNA) by Crithidia luciliae indirect immunofluorescence test (CLIFT) is reported qualitatively (positive or negative). For semiquantitative assessment of IgG antibodies to dsDNA, see results from ADNA / Double-stranded Antibodies, IgG, Serum.
A weak positive result dsDNA IgG by enzyme-linked immunosorbent assay with a CLIFT negative result may be suggestive of early disease, inactive disease, or a false positive result.
A positive result for IgG antibodies to dsDNA by Crithidia luciliae may occur in patients with diseases other than systemic lupus erythematosus (SLE).
A negative result does not exclude a diagnosis of SLE.
1. Aringer M, Costenbader K, Daikh D, et al: European league against rheumatism/American College of Rheumatology Classification Criteria for systemic lupus erythematosus. Arthritis Rheumatol. 2019 Sep;71(9):1400-1412. doi: 10.1002/art.40930
2. Petri M, Orbai AM, Alarcón GS, et al: Derivation and validation of the Systemic Lupus International Collaborating Clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum. 2012 Aug;64(8): 2677-2686. doi: 10.1002/art.34473
3. Enocsson H, Sjowall C, Wirestam L, et al: Four anti-dsDNA antibody assays in relation to systemic lupus erythematosus disease specificity and activity. J Rheumatol. 2015 May;42(5):817-825. doi: 10.3899/jrheum.140677
4. Sarbu MI, Salman-Monte TC, Munoz PR, Lisbona MP, Bernabe MA, Carbonell J: Differences between clinical and laboratory findings in patients with recent diagnosis of SLE according to the positivity of anti-dsDNA by the Crithidia luciliae method. Lupus. 2015 Oct;24(11):1198-1203. doi: 10.1177/0961203315573852
5. Rekvig OP: Autoimmunity and SLE: factual and semantic evidence-based critical analyses of definitions, etiology, and pathogenesis. Front Immunol. 2020 Oct 6;11:569234. doi: 10.3389/fimmu.2020.569234
6. Damoiseaux J, Andrade LEC, Carballo OG, et al: Clinical relevance of HEp-2 indirect immunofluorescent patterns: the International Consensus on ANA patterns (ICAP) perspective. Ann Rheum Dis. 2019 Jul;78(7):879-889. doi: 10.1136/annrheumdis-2018-214436
7. Bragazzi NL, Watad A, Damiani G, Adawi M, Amital H, Shoenfeld Y: Role of anti-DNA auto-antibodies as biomarkers of response to treatment in systemic lupus erythematosus patients: hypes and hopes. Insights and implications from a comprehensive review of the literature. Expert Rev Mol Diagn. 2019 Nov;19(11):969-978. doi: 10.1080/14737159.2019.1665511
8. Fox BJ, Hockley J, Rigsby P, Dolman C, Meroni PL, Ronnelid J: A WHO Reference Reagent for lupus (anti-dsDNA) antibodies: international collaborative study to evaluate a candidate preparation. Ann Rheum Dis. 2019 Dec;78(12):1677-1680. doi: 10.1136/annrheumdis-2019-215845
9. Choi MY, Clarke AE, St Pierre Y, et al: Antinuclear antibody-negative systemic lupus erythematosus in an international inception cohort. Arthritis Care Res (Hoboken). 2019 Jul;71(7):893-902. doi: 10.1002/acr.23712
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
86255
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
CRITH | dsDNA Ab by Crithidia IFA, IgG, S | 58466-4 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
62925 | dsDNA Ab by Crithidia IFA, IgG, S | 58466-4 |
37268 | Crithidia Interpretation | 69048-7 |