As an adjunct in the diagnosis of extraintestinal, invasive amebiasis
Enzyme-Linked Immunosorbent Assay (ELISA)
Amebiasis
Amebic Antibody
Amebic Serology
Amoeba Histolytica
Amoeba, Serum
Amoebiasis Antibody
E Histolytica Antibody, Serum
Entamoeba Histolytica Antibodies
Entamoeba Histolytica Serology
Parasite Serologies-done by clinic
Serum
Direct detection of Entamoeba histolytica in fecal specimens is recommended to diagnose intestinal amebiasis. See OAP / Ova and Parasite, Concentrate and Permanent Smear, Microscopy, Feces or OAPNS / Ova and Parasite, Microscopy, Varies.
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
0.4 mL
Gross hemolysis | Reject |
Gross lipemia | Reject |
Heat-inactivated specimen | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Frozen (preferred) | 30 days | |
Refrigerated | 7 days |
As an adjunct in the diagnosis of extraintestinal, invasive amebiasis
Amebiasis is an infection caused by the protozoan parasite, Entamoeba histolytica. The infection is acquired by ingestion of cysts in fecally contaminated food or water; excystation and infection occur in the large intestine. After excystation, trophozoites attach to the intestinal wall and excrete extracellular enzymes that enable invasion of the mucosa and spread to other organs, especially the liver and lung where abscesses may develop.
Amebiasis (or amebic dysentery) can cause bloody diarrhea accompanied by fever and prostration. White and red blood cells are found in the stool. Liver abscess can develop several weeks to months later producing hepatomegaly and fever.
Serology may be particularly useful in supporting the diagnosis of invasive disease with E histolytica, which is most commonly associated with amebic liver abscess. Serology should not be used to identify or diagnose amebic dysentery due to poor sensitivity in acute, noninvasive disease.
Negative
Reference values apply to all ages.
Negative: No antibodies to Entamoeba histolytica detected. This assay is intended for assessment of invasive amebiasis. Repeat testing in 2 to3 weeks if clinically indicated.
Equivocal: Recommend follow-up testing in 10 to 14 days if clinically indicated.
Positive: Results are suggestive of current or past infection with Entamoeba histolytica. Direct detection of E histolytica in stool or other specimen sources is recommended to diagnose acute amebiasis.
Previous episodes of intestinal amebiasis may produce a positive serology.
Serologic results should be used as an aid in diagnosis and should not be interpreted as diagnostic by themselves.
1. Bruckner DA: Amebiasis. Clin Microbiol Rev. 1992 Oct;5(4):356-369. doi: 10.1128/CMR.5.4.356
2. Petri WA, Haque R, Moonah SN: Entamoeba species, including amebic colitis and liver abscess. In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:3273-3286
Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second step by using enzyme-labelled protein A (the conjugate). The enzyme converts the colorless substrate (urea peroxide/TMB) to a blue end product. The enzyme reaction is stopped by adding sulfuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength greater than or equal to 620 nm.(Package insert: RIDASCREEN Entamoeba histolytica IgG. R-Biopharm AG; 07/10/2019)
Tuesday, Thursday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
86753
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
EHOLG | E. histolytica Ab, IgG, S | 22285-1 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
EHOLG | E. histolytica Ab, IgG, S | 22285-1 |
Change Type | Effective Date |
---|---|
New Test | 2023-01-26 |