Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas using client specified probes
Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphoma
Evaluating specimens in which standard cytogenetic analysis is unsuccessful
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
TLPMB | Probe, Each Additional (TLPMF) | No | No |
This test includes a charge for application of the first probe set (2 fluorescence in situ hybridization [FISH] probes) and professional interpretation of results. Analysis charges will be incurred based on the number of cells analyzed per probe set.
When specified, any of the following probes will be performed:
14q32.1 rearrangement, TCL1A break-apart
14q11.2 rearrangement, TRAD break-apart
i(7q), D7Z1/D7S486
+8, D8Z2/MYC
Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm is available, see Special Instructions.
Fluorescence In Situ Hybridization (FISH)
+8 (trisomy 8)
Hepatosplenic T-cell Lymphoma
inv(14) - inversion 14
Iso(7q) - isochromosome 7q
T-cell Prolymphocytic Leukemia (T-PLL)
TCL1A (14q32) rearrangement
TRAD (14q11.2) rearrangement
This test includes a charge for application of the first probe set (2 fluorescence in situ hybridization [FISH] probes) and professional interpretation of results. Analysis charges will be incurred based on the number of cells analyzed per probe set.
When specified, any of the following probes will be performed:
14q32.1 rearrangement, TCL1A break-apart
14q11.2 rearrangement, TRAD break-apart
i(7q), D7Z1/D7S486
+8, D8Z2/MYC
Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm is available, see Special Instructions.
Varies
This test is intended for instances when limited T-cell lymphoma fluorescence in situ hybridization (FISH) probes are needed. The FISH probes to be analyzed must be specified on the request, otherwise test processing may be delayed in order to determine intended analysis. If specific probes are not included with this test request, the test may be canceled and automatically reordered by the laboratory as TLPDF / T-Cell Lymphoma, Diagnostic FISH, Varies.
If the entire T-cell lymphoma panel is preferred, order TLPDF / T-Cell Lymphoma, Diagnostic FISH, Varies.
For patients with T-cell acute lymphoblastic leukemia/lymphoma (T-ALL/LBL), order either TALAF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Adult, Varies. or TALPF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Pediatric, Varies depending on the age of the patient.
For testing paraffin-embedded tissue samples from patients with T-lymphoblastic lymphoma (T-BLB), see TLBLF / T-Lymphoblastic Leukemia/Lymphoma, FISH, Tissue.
Advise Express Mail or equivalent if not on courier service.
1. A list of probes requested for analysis is required. Probes available for this test are listed in the Testing Algorithm section.
2. A reason for testing and a flow cytometry and/or a bone marrow pathology report should be sent with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
Question ID | Description | Answers |
---|---|---|
GC141 | Reason for Referral | |
GC142 | Probes Requested | |
GC143 | Specimen |
Whole blood ACD Bone marrow ACD Whole blood Na Hep Bone marrow Na Hep Whole blood EDTA Bone marrow EDTA |
Submit only 1 of the following specimens:
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 2-3 mL
Collection Instructions:
1. It is preferable to send the first aspirate from the bone marrow collection.
2. Invert several times to mix bone marrow.
Acceptable
Specimen Type: Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Invert several times to mix blood.
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Blood: 2 mL
Bone Marrow: 1 mL
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Ambient (preferred) | ||
Refrigerated |
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas using client specified probes
Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphoma
Evaluating specimens in which standard cytogenetic analysis is unsuccessful
This test includes a charge for application of the first probe set (2 fluorescence in situ hybridization [FISH] probes) and professional interpretation of results. Analysis charges will be incurred based on the number of cells analyzed per probe set.
When specified, any of the following probes will be performed:
14q32.1 rearrangement, TCL1A break-apart
14q11.2 rearrangement, TRAD break-apart
i(7q), D7Z1/D7S486
+8, D8Z2/MYC
Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm is available, see Special Instructions.
T-cell neoplasms are relatively uncommon, accounting for approximately 12% of all non-Hodgkin lymphomas. There are several subtypes of T-cell neoplasms: T-lymphoblastic leukemia , T-cell prolymphocytic leukemia , T-cell large granular lymphocytic leukemia , anaplastic large cell lymphoma , peripheral T-cell lymphoma, and various other cutaneous, nodal, and extranodal lymphoma subtypes.
There are a few common chromosome abnormalities associated with specific T-cell lymphoma subtypes evaluated by this FISH test as seen in the following table.
Table. Common Chromosome Abnormalities in T-Cell Lymphomas
Lymphoma subtype | Chromosome abnormality | Fluorescence in situ hybridization probe |
T-cell prolymphocytic leukemia/lymphoma (T-PLL) | inv(14)(q11q32) and t(14;14)(q11;q32) | 5'/3'TCL1A |
Reflex: 14q11.2 rearrangement | 5'/3'TRAD | |
Hepatosplenic T-cell lymphoma | Isochromosome 7q | D7S486/D7Z1 |
Trisomy 8 | D8Z2/MYC |
These probes have diagnostic relevance and can also be used to track response to therapy.
An interpretive report will be provided.
A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.
Detection of an abnormal clone supports a diagnosis of a T-cell lymphoma. The specific abnormality detected may help subtype the neoplasm.
The absence of an abnormal clone does not rule out the presence of lymphoma.
This test is not approved by the U.S. Food and Drug Administration, and it is best used as an adjunct to existing clinical and pathologic information.
Bone marrow is the preferred sample type for this fluorescence in situ hybridization test. If bone marrow is not available, a blood specimen may be used if there are neoplastic cells in the blood specimen (as verified by a hematopathologist).
1. Swerdlow S, Campo E, Harris NL, et al, eds: WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. IARC Press; 2017
2. Gesk S, Martin-Subero JI, Harder L, et al: Molecular cytogenetic detection of chromosomal breakpoints in T-cell receptor gene loci. Leukemia. 2003;17:738-745
3. Chin M, Mugishima H, Takamura M, et al: Hemophagocytic syndrome and hepatosplenic (gamma)(delta) T-cell lymphoma with isochromosome 7q and 8 trisomy. J Pediatr Hematol Oncol. 2004;26(6):375-378
This test is performed using commercially available and laboratory-developed probes. Rearrangements involving TCL1A and TRAD are detected using a dual-color break-apart strategy probe. Trisomy of chromosome 8 and isochromosome 7q are detected using enumeration strategy probes. For each probe set, 100 interphase nuclei are scored. All results are expressed as the percent abnormal nuclei.(Unpublished Mayo method)
Monday through Friday
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
88271x2, 88275 x1, 88291x1- FISH Probe, Analysis, Interpretation; 1 probe set
88271x2, 88275 x1 - FISH Probe, Analysis; each additional probe set (if appropriate)
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
TLPMF | T-cell Lymphoma B/BM, Spec FISH | In Process |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
614348 | Result Summary | 50397-9 |
614349 | Interpretation | 69965-2 |
614350 | Result Table | 93356-4 |
614351 | Result | 62356-1 |
GC141 | Reason for Referral | 42349-1 |
GC142 | Probes Requested | 78040-3 |
GC143 | Specimen | 31208-2 |
614352 | Source | 31208-2 |
614353 | Method | 85069-3 |
614354 | Additional Information | 48767-8 |
614355 | Disclaimer | 62364-5 |
614356 | Released By | 18771-6 |
Change Type | Effective Date |
---|---|
New Test | 2021-12-13 |