Test Catalog

Test Id : BETV2

BET v2 (Profilin), IgE, Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

Evaluation of patients suspected birch pollen allergy

 

Evaluation of patients with suspected peanut allergy

 

Evaluation of patients with oral allergy syndrome to other pollens or plant-based foods

Highlights

This test determines the relative amount of IgE antibody to the cross-reactive profilin, Bet v2.

 

IgE antibodies to Bet v2 are associated with birch pollen sensitivity and oral allergy syndrome.

 

The presence of IgE antibodies to Bet v2 represents a potential minor marker of peanut allergenicity.

 

Antibodies to Bet v2 may be associated with broad allergenic cross-reactivity with other profilin containing pollens or foods.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Fluorescence Enzyme Immunoassay (FEIA)

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

BET v2 (Profilin), IgE, S

Aliases
Lists additional common names for a test, as an aid in searching

Profilin

Betula verrucosa

Birch

Peanut

Specimen Type
Describes the specimen type validated for testing

Serum

Ordering Guidance

For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies in Special Instructions

 

BET v2 is also available as part of the PEANT / Peanut, IgE with Reflex to Peanut Components, IgE, Serum. This reflex test includes Ara h2, h1, h3, h6, h9, and Bet v2 (profilin) if the initial Peanut IgE test is detectable.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 0.5 mL for every 5 allergens requested

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

For 1 allergen: 0.3 mL

For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
Frozen 90 days
Ambient 7 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Evaluation of patients suspected birch pollen allergy

 

Evaluation of patients with suspected peanut allergy

 

Evaluation of patients with oral allergy syndrome to other pollens or plant-based foods

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Immunoglobulin E antibodies to the Bet v 2a profilin protein have been reported in 10% to 38% of birch pollen-allergic patients. Birch pollen is highly allergenic and is a significant cause of immediate hypersensitivity, affecting as much as 5% to 50% of the population of Western Europe. The presence of antibodies to Bet v 2, may also indicate sensitivity to other profilin containing pollens including ragweed pollen, mugwort pollen, and timothy grass pollen.

 

The profilin Bet v2 is related to, and cross-reactive with, antibodies to the potential peanut allergen profilin Ara h5. As profilin proteins are present in many other foods, sensitivity to profilin Bet v2 may be associated in broad allergen cross-reactivity among foods, including mango, peach, apple, hazelnut, celery, carrot, paprika, anise, fennel, coriander, cumin, tomato, and potato.

 

The most common manifestation of allergy to food in profilin related allergic individuals is oral allergy syndrome. Profilins are generally not resistant to heat and digestion. Individuals with birch pollen allergy and oral allergy syndrome are more frequently allergic to apples and peaches than to other foods. In cases of allergic reaction associated with oral allergy syndrome, rhinitis, itching, tingling, and other mild reactions on the oropharyngeal mucosa were reported to be the most common complaints.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Class

IgE kU/L

Interpretation

0

<0.10

Negative

0/1

0.10-0.34

Borderline/Equivocal

1

0.35-0.69

Equivocal

2

0.70-3.49

Positive

3

3.50-17.4

Positive

4

17.5-49.9

Strongly positive

5

50.0-99.9

Strongly positive

6

> or =100

Strongly positive

Concentrations > or =0.70 kU/L (Class 2 and above) will flag as abnormally high.

Interpretation
Provides information to assist in interpretation of the test results

Profilins are potentially cross-reactive allergenic proteins found in many plant pollens and tissues. IgE antibodies to the profilin Bet v2, while associated with birch pollen sensitivity, also represent a minor peanut allergen marker as it is cross-reactive with the peanut profilin Ara h5. The presence of antibodies to profilin Bet v2 is typically associated with milder allergic reactions and oral allergy syndrome.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Results from IgE antibody testing must be interpreted in the context of patient’s clinical evaluation and history of allergen exposures.

 

The major allergen in birch pollen is Bet v1 (which is homologous to the peanut allergen Ara h8), as antibodies to the Bet v1 is found in as many as 95% in birch pollen patients. In cases of suspected birch pollen allergy, testing for antibodies to the profilin Bet v1 should be considered in addition to this test for profilin Bet v2 antibodies.

 

Positive results for IgE to peanut allergy markers, such as profilin Bet v2, are not diagnostic for peanut allergy and only indicate that the patient may be sensitized to peanut component or a cross-reactive allergen. Clinical correlation of results from in vitro IgE testing with patient history of allergic or anaphylactic responses to peanut is recommended.

 

Testing for IgE antibodies may not be useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients in whom the medical management does not depend upon identification of allergen specificity.

 

False-positive results for IgE antibodies may occur in patients with markedly elevated serum IgE (>2500 kU/L) due to nonspecific binding to allergen solid phases.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Anhoej C, Backer V, Nolte H: Diagnostic evaluation of grass- and birch-allergic patients with oral allergy syndrome. Allergy. 2001 Jun;56(6):548-552. doi: 10.1034/j.1398-9995.2001.056006548.x

2. Sekerkova A, Polackova M: Detection of Bet v1, Bet v2 and Bet v4 specific IgE antibodies in the sera of children and adult patients allergic to birch pollen: evaluation of different IgE reactivity profiles depending on age and local sensitization. Int Arch Allergy Immunol. 2011;154(4):278-85. doi: 10.1159/000321819

3. D'Amato G, Cecchi L, Bonini S, et al: Allergenic pollen and pollen allergy in Europe. Allergy. 2007 Sep;62(9):976-990. doi: 10.1111/j.1398-9995.2007.01393.x

4. Cabanos C, Tandang-Silvas MR, Odijk V, et al: Expression, purification, cross-reactivity and homology modeling of peanut profilin. Protein Expr Purif. 2010 Sep;73(1):36-45. doi: 10.1016/j.pep.2010.03.005

5. Bublin M, Breiteneder H: Cross-reactivity of peanut allergens. Curr Allergy Asthma Rep. 2014 Apr;14(4):426. doi: 10.1007/s11882-014-0426-8

6. Chan ES, Greenhawt MJ, Fleischer DM, Caubet JC: Managing cross-reactivity in those with peanut allergy. J Allergy Clin Immunol Pract. 2019 Feb;7(2):381-386. doi: 10.1016/j.jaip.2018.11.012

7. Simberloff T, Parambi R, Bartnikas LM, et al: Implementation of a standardized clinical assessment and management plan (SCAMP) for food challenges. J Allergy Clin Immunol Pract. 2017 Mar-Apr;5(2):335-344.e3. doi:10.1016/j.jaip.2016.05.021

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

Specific IgE from the patient's serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti IgE is washed away and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA. Rev 06/2019)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 3 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

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  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

86008

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports