Assessing the nutrition intake of animal protein
The calculation of urinary supersaturation of various crystals or stones
High-Pressure Ion Chromatography (HPIC)
Sulfate
Urine
24-Hour volume is required.
Question ID | Description | Answers |
---|---|---|
TM89 | Collection Duration | |
VL71 | Urine Volume |
Supplies: Sarstedt 5 mL Aliquot Tube (T914)
Container/Tube: Plastic, 5-mL urine tube
Specimen Volume: 4 mL
Collection Instructions:
Collect urine for 24 hours.
2. No preservative.
3. Specimen must be kept refrigerated during and after collection.
4. Specimen pH should be between 4.5 and 8 and will stay in this range if kept refrigerated. Specimens with pH >8 indicate bacterial contamination, and testing will be cancelled. Do not attempt to adjust pH as it will adversely affect results.
Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.
Note: The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection.
Ambient | No |
Refrigerate | Preferred |
Frozen | OK |
50% Acetic Acid | No |
Boric Acid | OK |
Diazolidinyl Urea | OK |
6M Hydrochloric Acid | No |
6M Nitric Acid | No |
Sodium Carbonate | OK |
Thymol | OK |
Toluene | No |
1 mL
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Urine | Refrigerated (preferred) | 14 days | |
Frozen | 14 days |
Assessing the nutrition intake of animal protein
The calculation of urinary supersaturation of various crystals or stones
Urinary sulfate is a reflection of dietary protein intake, particularly meat, fish,
Sulfate is a major anion in the urine that has significant affinity for cations and
7-47 mmol/24 hours
Urinary sulfate is a reflection of dietary protein intake, particularly of
It also is used in the calculation of urinary supersaturation of various
No significant cautionary statements
1. Rodgers A, Gauvin D, Edeh S, et al: Sulfate but not thiosulfate reduces calculated and measured urinary ionized calcium and supersaturation: implications for the treatment of calcium renal stones. PLoS ONE. 2014;9(7):e103602 doi: 10.1371/journal.pone.0103602
2. Magee EA, Curno R, Edmond LM, Cummings JH: Contribution of dietary protein and inorganic sulfur to urinary sulfate: toward a biomarker of inorganic sulfur intake. Am J Clin Nutr 2004;80(1):137-142
3. Houterman S, van Faassen A, Ocke MC, et al: Is urinary sulfate a biomarker for the intake of animal protein and meat? Cancer Lett 1997;114:295-296
A high-pressure ion chromatography system (HPICS) utilizes an anion exchange column to separate sulfate from other anions present in urine. Detection of sulfate is done by conductivity change as eluent flows through a conductivity cell of a conductivity detector. The Thermo IC system is operated through a PC computer using thermo Chromeleon 7 software.(Christison T, Lopez L: Determination of Inorganic Anions in Acid Rain Using a Dedicated High-Pressure Capillary Ion Chromatography System. Technical Note 124 Thermo Scientific, 2016)
Monday through Friday
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
84392
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
SULFU | Sulfate, 24 Hr, U | 26889-6 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
SLF_U | Sulfate, 24 Hr, U | 26889-6 |
TM89 | Collection Duration | 13362-9 |
VL71 | Urine Volume | 3167-4 |
SUL_C | Sulfate Concentration, 24 Hr, U | 12920-5 |