An adjunct in the evaluation of menstrual irregularities
Evaluating patients with suspected hypogonadism
Predicting ovulation
Evaluating infertility
Diagnosing pituitary disorders
Electrochemiluminescence Immunoassay
Follicle Stimulating Hormone (FSH), Serum
FSH (Follicle-Stimulating Hormone)
FSH, Serum
Pituitary Gonadotropins
Serum
Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.6 mL
Collection Instructions: Centrifuge and aliquot serum within 2 hours of collection.
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen
0.5 mL
Gross hemolysis | Reject |
Gross lipemia | OK |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 7 days | |
Frozen | 180 days | ||
Ambient | 24 hours |
An adjunct in the evaluation of menstrual irregularities
Evaluating patients with suspected hypogonadism
Predicting ovulation
Evaluating infertility
Diagnosing pituitary disorders
Luteinizing hormone (LH) is a glycoprotein hormone consisting of 2 noncovalently bound subunits (alpha and beta). Gonadotropin-releasing hormone from the hypothalamus controls the secretion of the gonadotropins, follicle-stimulating hormone (FSH), and LH from the anterior pituitary.
The menstrual cycle is divided by a midcycle surge of both FSH and LH into a follicular phase and a luteal phase.
FSH appears to control gametogenesis in both males and females.
Males
<12 months: < or =3.3 IU/L
> or =12 months-< or =5 years: < or =1.9 IU/L
>5 years-< or =10 years: < or =2.3 IU/L
>10 years-< or =15 years: 0.6-6.9 IU/L
>15 years-< or =18 years: 0.7-9.6 IU/L
>18 years: 1.2-15.8 IU/L
TANNER STAGES*
Stage l: <1.5 IU/L
Stage ll: <3.0 IU/L
Stage lll: 0.4-6.2 IU/L
Stage lV: 0.6-5.1 IU/L
Stage V: 0.8-7.2 IU/L
*Puberty onset occurs for boys at a median age of 11.5 (+/- 2) years. For boys there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
Females
<12 months: 1.2-12.5 IU/L
> or =12 months-< or =10 years: 0.5-6.0 IU/L
>10 years-< or =15 years: 0.9-8.9 IU/L
>15 years-< or =18 years: 0.7-9.6 IU/L
Premenopausal:
Follicular: 2.9-14.6 IU/L
Midcycle: 4.7-23.2 IU/L
Luteal: 1.4-8.9 IU/L
Postmenopausal: 16.0-157.0 IU/L
TANNER STAGES*
Stage l: 0.6-4.1 IU/L
Stage ll: 0.3-5.8 IU/L
Stage lll: 0.1-7.2 IU/L
Stage lV: 0.3-7.0 IU/L
Stage V: 0.4-8.6 IU/L
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (+/- 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
For SI unit Reference Values, see https://www.mayocliniclabs.com/order-tests/si-unit-conversion.html
In both males and females, primary hypogonadism results in an
FSH and LH are generally elevated in:
-Primary gonadal failure
-Complete testicular feminization syndrome
-Precocious puberty (either idiopathic or secondary to a central
-Menopause (postmenopausal FSH levels are generally >40 IU/L)
-Primary ovarian hypofunction in females
-Primary hypogonadism in males
Normal or decreased FSH in:
-Polycystic ovary disease in females
FSH and LH are both decreased in failure of the pituitary or hypothalamus.
No clinically significant cross-reactivity has been demonstrated with thyrotropin (previously known as thyroid-stimulating hormone), luteinizing hormone, human chorionic gonadotropin, prolactin, or growth hormone.
Some patients who have been exposed to animal antigens, either in the environment or as part of treatment or imaging procedures, may have circulating anti-animal antibodies present. These antibodies may interfere with the assay reagents to produce unreliable results.
1. Demers LM, Vance ML: Pituitary function. In: Burtis CA, Ashwood ER, Bruns DE, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 4th ed. Elsevier Saunders Company; 2006:1984-1989
2. Haymond S, Gronowski AM: Reproductive related disorders. In: Burtis CA, Ashwood ER, Bruns DE, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 4th ed. Elsevier Saunders Company; 2006:2101-2127
3. Kulasingam V, Jung BP, Blastuig IM, et al: Pediatric reference intervals for 28 chemistries and immunoassays on the Roche cobas 6000 analyzer--A CALIPER pilot study. Clin Biochem. 2010 Sep;43(13-14);1045-1050. doi: 10.1016/j.clinbiochem.2010.05.008
4. Konforte D, Shea JL, Kyriakopoulou L, et al: Complex biological pattern of fertility hormones in children and adolescents: a study of healthy children from the CALIPER cohort and establishment of pediatric reference intervals. Clin Chem. 2013 Aug;59(8):1215-1227. doi: 10.1373/clinchem.2013.204123
In the Roche follicle-stimulating hormone (FSH) assay, the determination of the FSH is made with the aid of a biotinylated monoclonal FSH-specific antibody and a monoclonal FSH-specific antibody labeled with a ruthenium complex to form a sandwich complex. After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell. Application of a voltage to the electrode then induces chemiluminescent emission that is measured by a photomultiplier.(Package insert: FSH. Roche Diagnostics; 09/2018)
Monday through Sunday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
83001
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
FSH | Follicle-Stim Hormone (FSH), S | 15067-2 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
FSH | Follicle-Stim Hormone (FSH), S | 15067-2 |