Aids in the identification of respiratory syncytial viral infection
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
ISTOI | ISH Initial, Tech Only | No | No |
ISTOA | ISH Additional, Tech Only | No | No |
For the initial technical component only in situ hybridization (ISH) stain performed, the appropriate bill only test ID will be reflexed and charged (ISTOI). For each additional technical component only ISH stain performed, an additional bill only test ID will be reflexed and charged (ISTOA).
In Situ Hybridization
ISRSVABISHTO
ISNEGRSVABISH
ISRNARSVABISH
For the initial technical component only in situ hybridization (ISH) stain performed, the appropriate bill only test ID will be reflexed and charged (ISTOI). For each additional technical component only ISH stain performed, an additional bill only test ID will be reflexed and charged (ISTOA).
TECHONLY
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Container/Tube: Immunostain Technical Only Envelope
Preferred: 4 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block
1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/
2. Clients ordering stains using a manual requisition form will not have access to digital images.
3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/#FAQ
If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
TECHONLY | Ambient (preferred) | ||
Refrigerated |
Aids in the identification of respiratory syncytial viral infection
For the initial technical component only in situ hybridization (ISH) stain performed, the appropriate bill only test ID will be reflexed and charged (ISTOI). For each additional technical component only ISH stain performed, an additional bill only test ID will be reflexed and charged (ISTOA).
Respiratory syncytial virus (RSV) causes a wheezing illness that especially affects young children. The virus infects alveolar pneumocytes and induces fusion of multiple cells, creating a "syncytial" multinucleated cell. This RSV probe sequence was designed to identify subgroups RSV-A and RSV-B. It has been verified to not cross react with the following viruses in formalin-fixed, paraffin-embedded (FFPE) tissue: adenovirus (ADV), BK virus (BKV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), hepatitis C virus (HCV), human herpes virus, type 8 (HHV-8), human papillomavirus (HPV) types 6, 11, 16, E6/E7, Helicobacter pylori (H pylori), herpes simplex virus (HSV) types 1 and 2, JC virus (JCV), parvovirus, toxoplasma, and varicella-zoster viruses (VZV).
This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.
The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Age of a cut paraffin section can affect staining quality. Stability thresholds vary widely among published literature. Best practice is for paraffin sections to be cut within 6 weeks.
1. Hall CB: Respiratory syncytial virus and parainfluenza virus. N Engl J Med. 2001 Jun 21;344(25):1917-1928
2. Essaidi-Laziosi M, Lyon M, Mamin A, et al: Journal of Virological Methods. 2016;235;9-14
3. User manual: RNAscope 2.5 VS Assay for Ventana DISCOVER ULTRA System BROWN, Document Number 322200-USM_ULT
In situ hybridization on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Monday through Friday
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
88365-TC, Primary
88364-TC, if additional ISH
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
RSVAB | Resp Syncytial Virus(RSV)ISH,T Only | Order only;no result |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
601949 | Resp Syncytial Virus(RSV)ISH,T Only | Bill only; no result |