Useful For
Suggests clinical disorders or settings where the test may be helpful
Detect HIV-1 coreceptor tropism; determine eligibility for CCR5 antagonist therapy such as Selzentry™ (maraviroc).
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Method Name
A short description of the method used to perform the test
Polymerase chain reaction (PCR) amplification and viral culture.
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Yes
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Trofile DNA Phenotypic Corecept Tro
Aliases
Lists additional common names for a test, as an aid in searching
FTROF
CCR5, CXCR4, CR5
Chemokine co-receptor
Selzentry, Maraviroc
Trofile DNA
Tropism DNA
Tropotype DNA
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Specimen Type
Describes the specimen type validated for testing
Whole Blood EDTA
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Specimen Type: Whole Blood
Container/Tube: Lavender-top (EDTA)
Specimen Volume: 4 mL
Collection Instructions: Draw 4 mL blood in a lavender-top (EDTA) tube(s), (Do not centrifuge.) Freeze and ship frozen.
To avoid delays in turnaround time when requesting multiple tests, please submit separate frozen specimens for each test requested.
Note: Trofile DNA is recommended for patients with undetectable viral loads.
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
Other | Insufficient volume; heparinized plasma; non frozen specimen |
Specimen Type |
Temperature |
Time |
Special Container |
Whole Blood EDTA |
Frozen |
14 days |
|
Useful For
Suggests clinical disorders or settings where the test may be helpful
Detect HIV-1 coreceptor tropism; determine eligibility for CCR5 antagonist therapy such as Selzentry™ (maraviroc).
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Interpretation
Provides information to assist in interpretation of the test results
Trofile DNA Viral Classification
CCR5 Tropic (R5) HIV-1:
Virus uses CCR5 to enter CD4+ cells.
CXCR4 Tropic (X4) HIV-1:
Virus uses CXCR4 to enter CD4+ cells.
DUAL /MIXED Tropic (D/M) HIV-1:
Dual-tropic viruses can use either CXCR4 or CCR5 to enter CD4+ cells. Mixed-tropic populations contain viruses with 2 or more tropisms.
Nonreportable:
Co-receptor tropism could not be determined. Common causes of nonreportable results are reduced viral fitness or compromised sample handling. Please note that Trofile DNA sample collection and handling instructions differ from Trofile and other Monogram assays.
Trofile uses the complete gp160 coding region of the HIV-1 envelope protein ensuring that all of the determinants of tropism are tested. Subtype is determined based on the HIV-1 gp41 envelope region.
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Method Description
Describes how the test is performed and provides a method-specific reference
Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry into CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4. Trofile DNA uses the complete gp160 coding region of the HIV-1 envelope protein ensuring that all of the determinants of tropism tested.
Trofile DNA meets the US standards for technical validation as established by the Clinical Laboratory Improvement Amendments. Trofile DNA is a single cycle pseudovirion based tropism assay that uses the complete gp160 coding region of HIV-1 to evaluate tropism. Instead of using HIV-1 RNA isolated from patient plasma, Trofile DNA uses cell associated viral DNA taken from whole blood cells infected with HIV. HIV-1 envelopes encoded by the viral DNA are tested in a cell-based viral infectivity assay in order to determine which co- receptor the HIV-1 virus population is capable of using: CCR5, CXCR4, or both, known as D/M (dual/mixed).
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
No
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
31 to 37 days
Monogram Biosciences, Inc
Test Setup Resources
Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.
Excel |
Pdf
SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.
SI Normal Reports |
SI Abnormal Reports