Test Catalog

Test Id : FFTRO

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Trofile DNA Co-Receptor Tropism Assay

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Detect HIV-1 coreceptor tropism; determine eligibility for CCR5 antagonist therapy such as Selzentry™ (maraviroc).

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Polymerase chain reaction (PCR) amplification and viral culture.

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Trofile DNA Phenotypic Corecept Tro

Aliases
Lists additional common names for a test, as an aid in searching

FTROF

CCR5, CXCR4, CR5

Chemokine co-receptor

Selzentry, Maraviroc

Trofile DNA

Tropism DNA

Tropotype DNA

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Specimen Type
Describes the specimen type validated for testing

Whole Blood EDTA

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

 

Specimen Type: Whole Blood

Container/Tube: Lavender-top (EDTA)

Specimen Volume: 4 mL

Collection Instructions: Draw 4 mL blood in a lavender-top (EDTA) tube(s), (Do not centrifuge.) Freeze and ship frozen.

To avoid delays in turnaround time when requesting multiple tests, please submit separate frozen specimens for each test requested.

Note: Trofile DNA is recommended for patients with undetectable viral loads.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

3 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Other Insufficient volume; heparinized plasma; non frozen specimen

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Whole Blood EDTA Frozen 14 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Detect HIV-1 coreceptor tropism; determine eligibility for CCR5 antagonist therapy such as Selzentry™ (maraviroc).

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Interpretation
Provides information to assist in interpretation of the test results

Trofile DNA Viral Classification

 

CCR5 Tropic (R5) HIV-1:

Virus uses CCR5 to enter CD4+ cells.

 

CXCR4 Tropic (X4) HIV-1:

Virus uses CXCR4 to enter CD4+ cells.

 

DUAL /MIXED Tropic (D/M) HIV-1:

Dual-tropic viruses can use either CXCR4 or CCR5 to enter CD4+ cells.  Mixed-tropic populations contain viruses with 2 or more tropisms.

 

Nonreportable:

Co-receptor tropism could not be determined.  Common causes of nonreportable results are reduced viral fitness or compromised sample handling.  Please note that Trofile DNA sample collection and handling instructions differ from Trofile and other Monogram assays.

 

Trofile uses the complete gp160 coding region of the HIV-1 envelope protein ensuring that all of the determinants of tropism are tested. Subtype is determined based on the HIV-1 gp41 envelope region.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry into CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4.  Trofile DNA uses the complete gp160 coding region of the HIV-1 envelope protein ensuring that all of the determinants of tropism tested.

 

Trofile DNA meets the US standards for technical validation as established by the Clinical Laboratory Improvement Amendments.  Trofile DNA is a single cycle pseudovirion based tropism assay that uses the complete gp160 coding region of HIV-1 to evaluate tropism.  Instead of using HIV-1 RNA isolated from patient plasma, Trofile DNA uses cell associated viral DNA taken from whole blood cells infected with HIV.  HIV-1 envelopes encoded by the viral DNA are tested in a cell-based viral infectivity assay in order to determine which co- receptor the HIV-1 virus population is capable of using: CCR5, CXCR4, or both, known as D/M (dual/mixed).

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Sunday through Saturday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

31 to 37 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Monogram Biosciences, Inc

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87999

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
FFTRO Trofile DNA Phenotypic Corecept Tro 53923-9
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
Z2283 Tropotype Result 53923-9
Z2284 Interpretation: Not Provided

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports

Test Update Resources

Change Type Effective Date
Test Changes - Method 2023-02-01