Test Catalog

Test Id : FBL

Fungal Culture, Blood

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis and treatment of the etiologic agents of fungemia

 

Select patient population that presents with signs and symptoms of sepsis, especially fever of unknown origin

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
D2F D2 Fungal Sequencing Identification No, Bill Only No
FUNA Fungal Ident Panel A No, Bill Only No
FUNB Fungal Ident Panel B No, Bill Only No
LCCI Ident Rapid PCR Coccidioides No, Bill Only No
LCHB Id, Histoplasma/Blastomyces PCR No, Bill Only No
RMALF Id MALDI-TOF Mass Spec Fungi No, Bill Only No
RMALY Id MALDI-TOF Mass Spec Yeast No, Bill Only No
LCCA Id, Candida auris Rapid PCR No, Bill Only No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, reflex testing may be performed at an additional charge.

Method Name
A short description of the method used to perform the test

Conventional Broth Culture/Macroscopic/Microscopic/Nucleic Acid Hybridization/D2 rDNA Gene Sequencing/Real-Time Polymerase Chain Reaction (rtPCR)/Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

 

Dimorphic Pathogen Identification Confirmation: Nucleic Acid Hybridization/D2 rDNA Gene Sequencing/rtPCR/MALDI-TOF MS

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Fungal Culture, Blood

Aliases
Lists additional common names for a test, as an aid in searching

Culture, Fungus

Culture, Yeast

Fungus Culture

Mold

Mycology Culture

Yeast Culture

50022-FBL

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, reflex testing may be performed at an additional charge.

Specimen Type
Describes the specimen type validated for testing

Whole blood

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube:

Preferred: Green top (heparin)

Acceptable: SPS/Isolator tube

Specimen Volume: 10 to 30 mL

Collection Instructions:

1. Send specimen in original tube. Do not aliquot.

2. If collecting in an Isolator tube, draw blood in tube, and send 8 mL of whole blood in the original Isolator tube.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

5 mL

Pediatric: 1.5 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

No specimen should be rejected.

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Whole blood Ambient (preferred) 7 days
Refrigerated 7 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis and treatment of the etiologic agents of fungemia

 

Select patient population that presents with signs and symptoms of sepsis, especially fever of unknown origin

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, reflex testing may be performed at an additional charge.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Due to the high mortality rate from fungemia, the expeditious detection and identification of fungi from the patient's blood can have great diagnostic prognostic importance. Risk factors for fungemia include, but are not limited to, extremes of age, immunosuppression, and those individuals with burns or indwelling intravascular devices.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Negative

If positive, notification is made as soon as the positive culture is detected or identified.

Interpretation
Provides information to assist in interpretation of the test results

Positive cultures of yeast and filamentous fungi are reported with the organism identification.

 

Positive cultures are usually an indication of infection and are reported as soon as detected. Correlation of culture results and the clinical situation is required for optimal patient management. A final negative report is issued after 30 days of incubation.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

No significant cautionary statements

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Reimer LG, Wilson ML, Weinstein MP: Update on detection of bacteremia and fungemia. Clin Microbiol Rev. 1997 Jul;10(3):444-465

2. Procop GW, Cockerill FR III, Vetter EA, Harmsen WS, Hughes JG, Roberts GD: Performance of five agar media for recovery of fungi from isolator blood cultures. J Clin Microbiol. 2000 Oct;38(10):3827-3829

3. Procop GW, Church DL, Hall GS, et al: Mycology. In: Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Walters Kluwer; 2017:1322-1416

Method Description
Describes how the test is performed and provides a method-specific reference

Blood is inoculated into MycoF Lytic (Becton Dickinson) and Isolator (Wampole) tubes. Continuously monitored blood culture instruments provide for the detection of bloodstream infections due to most Candida species and Cryptococcus species. The Isolator tube contains saponin to lyse the blood cells, enabling the release of intracellular organisms. Centrifugation generates a concentrated layer of organisms that is inoculated onto solid media for recovery of fungi and this appears to be the most sensitive method for recovery of Histoplasma capsulatum, other dimorphic fungi, and filamentous fungi.(Sutton DA: Specimen collection, transport, and processing: Mycology. In: Murray PR, Baron EJ, Jorgensen JH, et al. Manual of Clinical Microbiology. 9th ed. ASM Press; 2007:1728-1736)

 

Identification of fungi is based on colonial and microscopic morphology, MALDI-TOF mass spectrometry, nucleic acid hybridization probes, laboratory-developed real-time PCR assays and/or D2 rDNA gene sequencing, as applicable.(Babady NE, Buckwalter SP, Hall L, Le Febre KM, Binnicker MJ, Wengenack NL: Detection of Blastomyces dermatitidis and Histoplasma capsulatum from culture isolates and clinical specimens by use of real-time PCR. J Clin Microbiol. 2011 Sep;49[9]:3204-3208; Binnicker MJ, Buckwalter SP, Eisberner JJ, et al: Detection of Coccidioides species in clinical specimens by real-time PCR. J Clin Microbiol. 2007 Jan;45[1]:173-178; Dhiman N, Hall L, Wohlfiel SL, Buckwalter SP, Wengenack NL: Performance and cost analysis of matrix-assisted laser desorption ionization time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol. 2011 Apr:49[4];1614-1616; Hall L, Wohlfiel SL, Roberts GD: Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol. 2004 Feb;42[2]:622-626; Theel ES, Schmidt BH, Hall L, et al: Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2012 Sep;50[9]:3093-3095; Theel ES, Hall L, Mandrekar J, Wengenack NL: Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011 Dec;49[12]:4067-4071)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

30 to 33 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

24 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87015-Concentration (any type) for infectious agents

87103-Blood

87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)

87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)

87107-Fungal identification panel A (if appropriate)

87107-Fungal identification panel B (if appropriate)

87150-Identification rapid PCR coccidioides (if appropriate)

87150 x 2- Identification Histoplasma/Blastomyces, PCR (if appropriate)

87153-D2 fungal sequencing identification (if appropriate)

87150- Id, Candida auris Rapid PCR (if appropriate)

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports