Test Id : A1APP
Alpha-1-Antitrypsin Phenotype, Serum
Useful For
Suggests clinical disorders or settings where the test may be helpful
Identification of homozygous and heterozygous phenotypes of the alpha-1-antitrypsin deficiency
Profile Information
A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
A1AP2 | Alpha-1-Antitrypsin Phenotype | No | Yes |
AATP | Alpha-1-Antitrypsin, S | Yes, (Order AAT) | Yes |
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
For information see Alpha-1-Antitrypsin-A Comprehensive Testing Algorithm.
Method Name
A short description of the method used to perform the test
A1AP2: Isoelectric Focusing
AATP: Nephelometry
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
A1A Phenotyping
a-1-Antitrypsin, Phenotype
AAT Phenotyping
Alpha 1 Antitrypsin
Anti-Alpha-1-Trypsin
Antitrypsin
Antitrypsin, Phenotype
Pi Typing (Alpha-1-Antitrypsin Phenotyping)
Protease Inhibitor Allo Typing
Alpha-1-Antitrypsin Reflex
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
For information see Alpha-1-Antitrypsin-A Comprehensive Testing Algorithm.
Specimen Type
Describes the specimen type validated for testing
Serum
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 1.25 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Forms
If not ordering electronically, complete, print, and send 1 of the following with the specimen:
-Gastroenterology and Hepatology Test Request (T728)
-General Request (T239)
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
0.5 mL
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
Gross hemolysis | OK |
Gross lipemia | Reject |
Gross icterus | OK |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 28 days | |
Ambient | 28 days | ||
Frozen | 28 days |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Identification of homozygous and heterozygous phenotypes of the alpha-1-antitrypsin deficiency
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
For information see Alpha-1-Antitrypsin-A Comprehensive Testing Algorithm.
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Alpha-1-antitrypsin (A1A) is the most abundant serum protease inhibitor and inhibits trypsin and elastin, as well as several other proteases. The release of proteolytic enzymes from plasma onto organ surfaces and into tissue spaces results in tissue damage unless inhibitors are present. Congenital deficiency of A1A is associated with the development of emphysema at an unusually early age and with an increased incidence of neonatal hepatitis, usually progressing to cirrhosis.
The gene for A1A appears to be coded at a single locus whose alleles are inherited in a co-dominant manner. Most normal individuals have the M phenotype (M, M1, or M2). Over 99% of M phenotypes are genetically homozygous M (MM). In the absence of family studies, the phenotype (M) and quantitative level can be used to infer the genotype MM. The most common alleles associated with a quantitative deficiency are Z and S.
For more information see Alpha-1-Antitrypsin-A Comprehensive Testing Algorithm.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
ALPHA-1-ANTITRYPSIN
100-190 mg/dL
ALPHA-1-ANTITRYPSIN PHENOTYPE
The interpretive report will identify the alleles present. For rare alleles, the report will indicate whether they have been associated with reduced quantitative levels of alpha-1-antitrypsin.
Interpretation
Provides information to assist in interpretation of the test results
There are greater than 40 alpha-1-antitrypsin (A1A) phenotypes (most of these are associated with normal quantitative levels of protein). The most common normal phenotype is M (M, M1, or M2), and greater than 90% of individuals of European descent are genetically homozygous M (MM).
Alpha-1-antitrypsin deficiency is usually associated with the Z phenotype (homozygous ZZ), but SS and SZ are also associated with decreased A1A levels.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
This assay identifies the phenotype of the circulating alpha-1-antitrypsin (A1A) protein. If the patient is already on replacement therapy or has recently been transfused, the phenotype will detect patient and replacement or transfused plasma A1A protein. This test also cannot detect a null allele which could be responsible for an A1A deficiency.
If 2 bands are seen, such as an M band and a Z bands, it is reported as MZ (eg, heterozygous)
If 1 band is seen, such as the Z band and the quantitative level is consistent with a homozygote, the phenotype is assumed to be homozygous and is reported as ZZ.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Morse JO. Alpha-1-antitrypsin deficiency. N Engl J Med. 1978;299:1045-1048;1099(20) 1099-1105
2. Donato LJ, Jenkins SM, Smith C, et al. Reference and interpretive ranges for alpha(1)-antitrypsin quantitation by phenotype in adult and pediatric populations. Am J Clin Pathol.2012;138(3):398-405
3. Stoller JK, Lacbawan FL, Aboussouan SF: Alpha-1 antitrypsin deficiency. In: Adam MP, Ardinger HH, Pagon RA, et al, eds. GeneReviews.[Internet]. University of Washington, Seattle; 2006. Updated June 1, 2023. Accessed May 21, 2025. Available at www.ncbi.nlm.nih.gov/books/NBK1519/
4. Rosenberg W, Badrick T, Tanwar S: Liver disease. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:1348-1397
Method Description
Describes how the test is performed and provides a method-specific reference
Alpha-1-Antitrypsin Phenotype
Phenotyping is done by isoelectric focusing in agarose gels. Certain alleles are associated with various serum levels of alpha-1-antitrypsin, and their respective proteins exhibit differential mobility on electrophoresis.(Package insert: Hydragel 18 A1AT Isofocusing. Sebia. 01/2020)
Alpha-1-Antitrypsin
In this Siemens Nephelometer II method, the light scattered by the antigen-antibody complexes is measured. The intensity of the measured scattered light is proportional to the amount of antigen-antibody complexes in the sample under certain conditions. If the antibody volume is kept constant, the signal behaves proportionally to the antigen volume. A reference curve is generated by a standard with a known antigen content on which the scattered light signals of the samples can be evaluated and calculated as an antigen concentration. Antigen-antibody complexes are formed when a sample containing antigen and the corresponding antiserum are put into a cuvette. A light beam is generated with a light emitting diode (LED), which is transmitted through the cuvette. The light is scattered onto the immuno-complexes that are present. Antigen and antibody are mixed in the initial measurement, but no complex is formed yet. An antigen-antibody complex is formed in the final measurement. The result is calculated by subtracting value of the final measurement from the initial measurement. The distribution of intensity of the scattered light depends on the ratio of the particle size of the antigen-antibody complexes to the radiated wavelength.(Instruction manual: Siemens Nephelometer II. Siemens, Inc; V 2.4, 07/2019; Addendum to the Instruction Manual 2.3, 08/2017)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
82103
82104
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
A1APP | Alpha-1-Antitrypsin Phenotype | 32769-2 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
AATP | Alpha-1-Antitrypsin, S | 6771-0 |
8166 | Alpha-1-Antitrypsin Phenotype | 32769-2 |