Test Catalog

Test Id : ABTIR

Antibody Titer, Whole Blood and Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

Monitoring antibody levels during pregnancy to help assess the risk of hemolytic disease of the newborn

 

This test is not useful for monitoring the efficacy of Rh-immune globulin administration.

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
ABIDR Antibody Identification, RBC Yes No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

If the antibodies detected are too weakly reactive to titer, the Antibody Titer test will be canceled and replaced by the Antibody Identification test (ABIDR).

Method Name
A short description of the method used to perform the test

Hemagglutination

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Antibody Titer, RBC

Aliases
Lists additional common names for a test, as an aid in searching

ABTIR

Antibody Titer, RBC

Allo Titer

Antibody Titer, Erythrocytes

RBC Antibody Titer

Rh (Rhesus) Titer

Anti-D Titer

Anti-E Titer

Anti-C Titer

Anti-Fya Titer

Anti-Fyb Titer

Duffy Antibody Titer

Kell Antibody Titer

Anti-K Titer

Anti-Jka Titer

Anti-Jkb Titer

Kidd Antibody Titer

Anti-e

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

If the antibodies detected are too weakly reactive to titer, the Antibody Titer test will be canceled and replaced by the Antibody Identification test (ABIDR).

Specimen Type
Describes the specimen type validated for testing

Varies

Shipping Instructions

Specimen must arrive within 72 hours of draw.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Both blood and serum are required.

 

Specimen Type: Blood

Collection Container/Tube: 6-mL PINK-top (EDTA)

Submission Container/Tube: Aliquot tube

Specimen Volume:

3 mL plasma

3 mL RBCs

Collection Instructions: Spin down and separate plasma from cells. Send both tubes.

 

Specimen Type: Serum

Collection Container/Tube: 10-mL Red top

Submission Container/Tube: Aliquot tube

Specimen Volume:

5 mL serum

5 mL RBCs

Collection Instructions: Spin down and separate serum from clot. Send both tubes.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Blood: 6 mL EDTA

Pediatric: 2 mL serum

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

No specimen should be rejected.

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Varies Ambient (preferred) 4 days
Refrigerated 4 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Monitoring antibody levels during pregnancy to help assess the risk of hemolytic disease of the newborn

 

This test is not useful for monitoring the efficacy of Rh-immune globulin administration.

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

If the antibodies detected are too weakly reactive to titer, the Antibody Titer test will be canceled and replaced by the Antibody Identification test (ABIDR).

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Some maternal IgG alloantibodies to red blood cell antigens will cross the placenta and cause hemolysis of antigen-positive fetal red cells. The resulting fetal anemia and hyperbilirubinemia can be harmful or even fatal to the newborn.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Negative,

If positive, result will be reported as the reciprocal of the highest dilution at with macroscopic agglutination (1+) is observed.

Interpretation
Provides information to assist in interpretation of the test results

The specificity of the maternal alloantibody will be stated. The titer result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed.

 

If the antibody problem identified is not relevant in hemolytic disease of the newborn or if titrations are not helpful, the titer will be canceled and will be replaced by ABIDR / Antibody Identification, RBC. 

 

A consultation service is offered, at no charge, regarding the clinical relevance of red cell antibodies.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Recent administration of Rh-immune globulin may cause anti-D to be identified and appear falsely as an alloantibody.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

AABB Technical Manual. 19th edition. Edited by MK Fung, AF Eder, SL Spitalnik, CM Westhoff. AABB, 2017

Method Description
Describes how the test is performed and provides a method-specific reference

The strength and specificity of the antibody to be titered is first determined. Two-fold serial dilutions of serum are tested against antigen-positive erythrocytes. The result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed at the antihuman globulin phase of testing. Parallel titration of a previous specimen of the patient's serum (frozen) provides a baseline for comparison of antibody level. In the absence of a previous specimen from the patient, parallel titration of a control antiserum is used for standardization.(AABB Technical Manual. 19th edition. Edited by MK Fung, AF Eder, SL Spitalnik, CM Westhoff. AABB, 2017)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday, Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 2 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

86886-Antibody titer

86870-Antibody Identification (if appropriate-per panel tested)

86860-Antibody elution (if appropriate)

86880 x 3-Antigloblin, direct (if appropriate)

86905-Each red cell antigen typing (if appropriate)

86978-Adsorption, each (if appropriate)

81403-Human Erythrocyte Antigen (if appropriate)-Internal only

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports