Assessing positive pretransfusion antibody screens, transfusion reactions, hemolytic disease of the newborn, and autoimmune hemolytic anemias
This test is not useful for monitoring the efficacy of Rh-immune globulin administration.
This test is not useful for identifying antibodies detected only at 4 degrees C or only after extended room temperature incubation.
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
DCTR | Direct Antiglobulin Test (Poly) | Yes | No |
SPAGR | Special Red Cell Ag Typing | Yes | No |
HEA | Human Erythrocyte Antigen | No, - Internal only | No |
DC3TR | Direct Antiglobulin Test (C3) | No | No |
DIGTR | Direct Antiglobulin Test (IgG) | No | No |
STTX25 | Antibody Elution | No | No |
STTX31 | Antibody Adsorption | No | No |
STTX32 | Red Cell Antigen Typing | No | No |
DATR | Direct Antiglobulin Tst (Poly) | No | No |
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
STTX26 | Antibody Panel | No | Yes |
The following tests may also be ordered and performed as part of antibody identification: DCTR / Direct Antiglobulin Test (Polyspecific), Blood and its reflexes and SPAGR / Special Red Cell Antigen Typing, Whole Blood. Additional reflex charges will be added for Antibody Elution, Antibody Adsorption, Antibody Panels, Special Red Cell Antigen Typings and Human Erythrocyte Antigen (internal only).
Hemagglutination
ABIDR
Antibody Identification, RBC
Antibody Workup
Red Cell Antibody Identification
Antibody Panel
The following tests may also be ordered and performed as part of antibody identification: DCTR / Direct Antiglobulin Test (Polyspecific), Blood and its reflexes and SPAGR / Special Red Cell Antigen Typing, Whole Blood. Additional reflex charges will be added for Antibody Elution, Antibody Adsorption, Antibody Panels, Special Red Cell Antigen Typings and Human Erythrocyte Antigen (internal only).
Varies
Specimen must arrive within 72 hours of collection.
Both blood and serum are required.
Supplies: Sarstedt 5 mL Aliquot Tube (T914)
Specimen Type: Plasma/Blood
Collection Container/Tube: 6-mL Pink-top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume:
3 mL plasma
3 mL red blood cells (RBC)
Collection Instructions:
1. Centrifuge and aliquot plasma into plastic vial.
2. Label specimen as EDTA plasma or EDTA RBC, as appropriate.
3. Send both tubes.
Specimen Type: Serum/Blood
Collection Container/Tube: 10-mL Red top
Submission Container/Tube: Plastic vial
Specimen Volume:
5 mL serum
5 mL RBC
Collection Instructions:
1. Centrifuge and aliquot serum into plastic vial.
2. Label specimen as serum or clotted RBC, as appropriate.
3. Send both tubes.
Blood: 6 mL EDTA
Pediatric: 2 mL serum
Gross hemolysis | OK |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Ambient (preferred) | 4 days | |
Refrigerated | 4 days |
Assessing positive pretransfusion antibody screens, transfusion reactions, hemolytic disease of the newborn, and autoimmune hemolytic anemias
This test is not useful for monitoring the efficacy of Rh-immune globulin administration.
This test is not useful for identifying antibodies detected only at 4 degrees C or only after extended room temperature incubation.
The following tests may also be ordered and performed as part of antibody identification: DCTR / Direct Antiglobulin Test (Polyspecific), Blood and its reflexes and SPAGR / Special Red Cell Antigen Typing, Whole Blood. Additional reflex charges will be added for Antibody Elution, Antibody Adsorption, Antibody Panels, Special Red Cell Antigen Typings and Human Erythrocyte Antigen (internal only).
After exposure to foreign red blood cells (RBC) via transfusion or pregnancy, some people form antibodies that are capable of the destruction of transfused RBC or of fetal RBC in utero. It is important to identify the antibody specificity in order to assess the antibody's capability of causing clinical harm and, if necessary, to avoid the antigen on transfused RBC.
Autoantibodies react against the patient's own RBC as well as the majority of cells tested. Autoantibodies can be clinically benign or can hemolyze the patient's own RBC, such as in cold agglutinin disease or autoimmune hemolytic anemia.
Negative,
If positive, antibodies will be identified and corresponding special red cell antigen typing on patient’s red blood cells will be performed.
Specificity of alloantibodies will be stated.
The patient's red blood cells will be typed for absence of the corresponding antigens or as an aid to identification in complex cases.
A consultation service is offered, at no charge, regarding the clinical relevance of red cell antibodies.
Recent administration of Rh-immune globulin may cause anti-D to be identified and appear falsely as an alloantibody.
Fung MK, Eder AF, Spitalnik SL, Westhoff CM: Technical Manual. 19th ed. AABB; 2017
A panel of reagent type O erythrocytes, with known antigenic determinants and the patient's cells are tested with the patient's serum/plasma. This panel should yield a distinct pattern of agglutination or hemolysis that identifies the auto- or alloantibody specificity. Elution, absorption, neutralization, and other special techniques may be necessary to complete antibody identification.( Fung MK, Eder AF, Spitalnik SL, Westhoff CM: Technical Manual. 19th ed. AABB; 2017)
Monday through Friday, Sunday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
86870
86860- (if appropriate)
86880 x 3 (if appropriate)
86905- (if appropriate)
86978- (if appropriate)
81403- (if appropriate) - Internal only
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
ABIDR | Antibody Identification, RBC | 888-8 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
ABDR1 | Antibody Identification, RBC | 888-8 |