Test Catalog

Test Id : QUAD1

Quad Screen (Second Trimester) Maternal, Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

Prenatal screening for open neural tube defect (alpha-fetoprotein only), trisomy 21 (alpha-fetoprotein, human chorionic gonadotropin, estriol, and inhibin A) and trisomy 18 (alpha-fetoprotein, human chorionic gonadotropin, and estriol)

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Immunoenzymatic Assay

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

QUAD SCRN (2nd Tri) Maternal, S

Lists additional common names for a test, as an aid in searching

AFP 4 Marker Screen

AFP Maternal Screening

AFP Neural Tube Defects

E3 (Estriol)

Maternal Screening, AFP Four Marker


Triple Test

UE3 (Unconjugated Estriol)

Specimen Type
Describes the specimen type validated for testing


Necessary Information

In order to provide the best results, either answer the order entry questions or provide the required information using the Second Trimester Maternal Screening Alpha-Fetoprotein (AFP)/QUAD Screen Patient Information (T595).


Question ID Description Answers
DRPHN Physician Phone Number
ESTDD Patient's Estimated Due Date (EDD)
MTHOD Method Used to Determine EDD Ultrasound
MTWT Patient Weight
LBKGS Units (lbs or kg) lbs
IDD Insulin dependent diabetes None
RACE1 Patients race Black
MULTF Number of Fetuses 1
3 or more
CHOR_ Number of Chorions Monochorionic
Not applicable
IVFP IVF pregnancy No
EGGDR IVF Egg Donor Date of Birth
EGGFR IVF Egg or Embryo Freeze Date
PRHIS Prev Down (T21) / Trisomy Pregnancy No
If MAFP - Not applicable
PRNTD Prev Pregnancy w/ Neural Tube Defect No
PTNTD Patient or father of baby has a NTD No
SMKNG Current Cigarette smoking status Non-smoker
INTL Initial or repeat testing Initial

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing


Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Do not collect specimen after amniocentesis as this could affect results.

2. Centrifuge and aliquot serum into plastic vial within 2 hours of collection

Additional Information:

1. For an assessment that includes neural tube defect results, gestational age must be between 15 weeks, 0 days and 22 weeks, 6 days.

2. Assessments for trisomy 21 (Down syndrome) and trisomy 18 (Edwards syndrome) only are available between 14 weeks, 0 days and 22 weeks, 6 days.

3. Initial or repeat testing is determined in the laboratory at the time of report and will be reported accordingly. To be considered a repeat test for the patient, the testing must be within the same pregnancy and trimester, with interpretable results for the same tests, and both tests are performed at Mayo Clinic.

4. Maternal Serum Screening patient education brochure (T522) is available upon request.

Special Instructions
Library of PDFs including pertinent information and forms related to the test


Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.75 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
Frozen 90 days
Ambient 7 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Prenatal screening for open neural tube defect (alpha-fetoprotein only), trisomy 21 (alpha-fetoprotein, human chorionic gonadotropin, estriol, and inhibin A) and trisomy 18 (alpha-fetoprotein, human chorionic gonadotropin, and estriol)

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Maternal serum screening is used to identify pregnancies that may have an increased risk for certain birth defects, including neural tube defects (NTD), trisomy 21 (Down syndrome), and trisomy 18 (Edwards syndrome). The screen is performed by measuring analytes in maternal serum that are produced by the fetus and the placenta. The analyte values along with maternal demographic information such as age, weight, gestational age, diabetic status, and race are combined in a mathematical model to derive a risk estimate. A specific cutoff for each condition is used to classify the risk estimate as either screen-positive or screen-negative. A screen-positive result indicates that the value obtained exceeds the established cutoff. A positive screen does not provide a diagnosis but rather indicates that further evaluation should be considered.




Alpha-fetoprotein (AFP) is a fetal protein that is initially produced in the fetal yolk sac and liver. A small amount is produced by the gastrointestinal tract. By the end of the first trimester, nearly all of the AFP is produced by the fetal liver. The concentration of AFP peaks in fetal serum between 10 to 13 weeks. Fetal AFP diffuses across the placental barrier into the maternal circulation. A small amount also is transported from the amniotic cavity.


The AFP concentration in maternal serum rises throughout pregnancy, from a non-pregnancy level of 0.2 ng/mL to about 250 ng/mL at 32 weeks gestation. If the fetus has an open NTD, AFP is thought to leak directly into the amniotic fluid causing unexpectedly high concentrations of AFP. Subsequently, the AFP reaches the maternal circulation, thus producing elevated serum levels. Other fetal abnormalities such as omphalocele, gastroschisis, congenital renal disease, esophageal atresia, and other fetal distress situations (eg, threatened abortion and fetal demise) also may result in maternal serum AFP elevations. Increased maternal serum AFP concentrations also may be seen in multiple pregnancies and in unaffected singleton pregnancies in which the gestational age has been underestimated.


Lower maternal serum AFP concentrations have been associated with an increased risk for genetic conditions such as trisomy 21 and trisomy 18.



Estriol, the principal circulatory estrogen hormone in the blood during pregnancy, is synthesized by the intact feto-placental unit. Estriol exists in maternal blood as a mixture of the unconjugated form and a number of conjugates. The half-life of unconjugated estriol in the maternal blood system is 20 to 30 minutes because the maternal liver quickly conjugates estriol to make it more water soluble for urinary excretion. Estriol levels increase during the course of pregnancy. Decreased unconjugated estriol has been shown to be a marker for trisomy 21 and trisomy 18. Low levels of estriol also have been associated with pregnancy loss, Smith-Lemli-Opitz, and X-linked ichthyosis (placental sulfatase deficiency).


Human Chorionic Gonadotropin:

Human chorionic gonadotropin (hCG) is a glycoprotein consisting of 2 noncovalently bound subunits. The alpha subunit is identical to that of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyrotropin (TSH, formerly thyroid-stimulating hormone), while the beta subunit has significant homology to the beta subunit of LH and limited similarity to the FSH and TSH beta subunits. The beta subunit determines the unique physiological, biochemical, and immunological properties of hCG. hCG is synthesized by placental cells, starting very early in pregnancy, and serves to maintain the corpus luteum, and hence, progesterone production, during the first trimester. Thereafter, the concentration of hCG begins to fall as the placenta begins to produce steroid hormones and the role of the corpus luteum in maintaining pregnancy diminishes.


Increased total hCG levels are associated with trisomy 21, while decreased levels may be seen in trisomy 18. Elevations of hCG also can be seen in multiple pregnancies, unaffected singleton pregnancies in which the gestational age has been overestimated, triploidy, fetal loss, and hydrops fetalis.


Inhibin A:

Inhibins are a family of heterodimeric glycoproteins, primarily secreted by ovarian granulosa cells and testicular Sertoli cells, which consist of disulfide-linked alpha and beta subunits. While the alpha subunits are identical in all inhibins, the beta subunits exist in 2 major forms, termed A and B, each of which can occur in different isoforms. Depending on whether an inhibin heterodimer contains a beta A or a beta B chain, they are designated as inhibin A or inhibin B, respectively. Together with the related activins, which are homodimers or heterodimers of beta A and B chains, the inhibins are involved in gonadal-pituitary feedback and in paracrine regulation of germ cell growth and maturation. During pregnancy, inhibins and activins are produced by the feto-placental unit in increasing quantities, mirroring fetal growth. Their physiological role during pregnancy is uncertain. They are secreted into the coelomic and amniotic fluid, but only inhibin A is found in appreciable quantities in the maternal circulation during the first and second trimesters.


Maternal inhibin A levels are correlated with maternal hCG levels and are abnormal in the same conditions that are associated with abnormal hCG levels (eg, inhibin A levels are typically higher in trisomy 21 pregnancies). However, despite their similar behavior, measuring maternal serum inhibin A concentrations in addition to maternal serum hCG concentrations further improves the sensitivity and specificity of maternal multiple marker screening for trisomy 21.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.


An alpha-fetoprotein (AFP) multiple of the median (MoM) <2.5 is reported as screen negative.

AFP MoM > or =2.5 (singleton and twin pregnancies) are reported as screen positive.



Calculated screen risks <1/270 are reported as screen negative, risks > or =1/270 are reported as screen positive.



Calculated screen risks <1/100 are reported as screen negative, risks > or =1/100 are reported as screen positive.


An interpretive report will be provided.

Provides information to assist in interpretation of the test results

Neural Tube Defects

A screen-negative result indicates that the calculated alpha-fetoprotein (AFP) multiple of the median (MoM) falls below the established cutoff of 2.50 MoM. A negative screen does not guarantee the absence of neural tube defects (NTD).


A screen-positive result indicates that the calculated AFP MoM is 2.50 or greater and may indicate an increased risk for open NTD. The actual risk depends on the level of AFP and the individual's pretest risk of having a child with NTD based on family history, geographical location, maternal conditions such as diabetes and epilepsy, and use of folate prior to conception. A screen-positive result does not infer a definitive diagnosis of NTD but indicates that further evaluation should be considered. Approximately 80% of pregnancies affected with NTD have elevated AFP, MoM values greater than 2.5.


Trisomy 21 (Down syndrome) and Trisomy 18 (Edwards syndrome):

A screen-negative result indicates that the calculated screen risk is below the established cutoff of 1/270 for trisomy 21 and 1/100 for trisomy 18. A negative screen does not guarantee the absence of trisomy 21 or trisomy 18.


When a trisomy 21 second-trimester risk cutoff of 1/270 is used for follow-up, the combination of maternal age, AFP, estriol, human chorionic gonadotropin ,and inhibin A has an overall detection rate of approximately 77% to 81% with a false-positive rate of 6% to 7%. In practice, both the detection rate and false-positive rate increase with age. The detection rate ranges from 66% (early teens) to 99% (late 40s), with false-positive rates of between 3% and 62%, respectively.


The detection rate for trisomy 18 is 60% to 80% using a second trimester cutoff of 1/100.



Upon receiving maternal serum screening results, all information used in the risk calculation should be reviewed for accuracy (maternal date of birth, gestational dating, etc). If any information is incorrect, the laboratory should be contacted for a recalculation of the estimated risks.


Screen-negative results typically do not warrant further evaluation.


Ultrasound is recommended to confirm dates for NTD or trisomy 21 screen-positive results. Many pregnancies affected with trisomy 18 are small for gestational age. Recalculations that lower the gestational age may decrease the detection rate for trisomy 18. If ultrasound yields new dates that differ by at least 7 days, a recalculation should be considered. If dates are confirmed, high-resolution ultrasound and amniocentesis (including amniotic fluid AFP and acetylcholinesterase measurements for NTD) are typically offered.

Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Variables Affecting Marker Levels:

Race, weight, smoking, multiple fetus pregnancy, insulin-dependent diabetes (IDD), and in vitro fertilization (IVF) may affect marker concentrations. Black mothers tend to have higher alpha-fetoprotein (AFP) levels but lower risk of neural tube defects and are assigned to a separate AFP median set. All multiples of the median (MoM) are adjusted for maternal weight (to account for dilution effects in heavier mothers). The AFP, unconjugated estriol (uE3), and inhibin MoM are adjusted upward in IDD to account for lower values in diabetic pregnancies. Human chorionic gonadotropin (hCG) levels are higher and uE3 levels are lower in pregnancies conceived by IVF, MoM are adjusted accordingly to account for the alterations. Smoking results in higher second trimester maternal serum AFP and inhibin A levels and lower uE3 and hCG levels. MoM are adjusted accordingly to account for analyte differences in smokers.


The estimated risk calculations and screen results are dependent on accurate information for gestation, maternal age, race, IDD, and weight. Inaccurate information can lead to significant alterations in the estimated risk. In particular, erroneous assessment of gestational age can result in false-positive or false-negative screen results. Because of its increased accuracy, the determination of gestational age by ultrasound is recommended, when possible, rather than by last menstrual period.


A screen-negative result does not guarantee the absence of fetal defects. A screen-positive result does not provide a diagnosis but indicates that further diagnostic testing should be considered (an unaffected fetus may have screen-positive result for unknown reasons).


Valid measurements of AFP in maternal serum cannot be made after amniocentesis.


Triplet and higher multiple pregnancies cannot be interpreted.


Each center offering maternal serum screening to patients should establish a standard screening protocol that provides pre- and post-screening education and appropriate follow-up for screen-positive results.


In a small percentage of samples, there is potential for alkaline phosphatase associated positive interference in the Beckman Access uE3 assay. This potential interference does not appear to be related to the amount of alkaline phosphatase in the patient sample.  A falsely elevated uE3 test result can lead to inaccurately underestimating the relative risk of chromosomal abnormalities, such as trisomy 21 and 18.


In rare cases, some individuals can develop antibodies to mouse or other animal antibodies (often referred to as human anti-mouse antibodies [HAMA] or heterophile antibodies), which may cause interference in some immunoassays. Caution should be used in interpretation of results and the laboratory should be alerted if the result does not correlate with the clinical presentation.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Wald NJ, Cuckle HS, Densem JW, Stone RB: Maternal serum unconjugated oestriol and human chorionic gonadotrophin levels in pregnancies with insulin-dependent diabetes: implications for screening for Down's syndrome. Br J Obstet Gynaecol. 1992 Jan;99(1):51-53

2. American College of Obstetricians and Gynecologists: Practice Bulletin No. 163: Screening for Fetal Aneuploidy. Obstet Gynecol. 2016 May;127(5):e123-137

3. Malone FD, Canick JA, Ball RH, et al: First-trimester or second-trimester screening, or both, for Down's syndrome. N Engl J Med. 2005 Nov 10;353(19):2001-2011

4. Wald NJ, Rodeck C, Hackshaw AK, et al: SURUSS in perspective. Semin Perinatol. 2005 Aug(4);29:225-235

5. Rudnicka AR, Wald NJ, Huttly W, Hackshaw AK: Influence of maternal smoking on the birth prevalence of Down syndrome and on second trimester screening performance. Prenat Diagn. 2002 Oct;22(10):893-897

6. Zhang J, Lambert-Messerlian G, Palomaki GE, Canick JA: Impact of smoking on maternal serum markers and prenatal screening in the first and second trimesters. Prenat Diagn. 2011 Jun;31(6):583-588

7. Yarbrough ML, Stout M, Gronowski AM: Pregnancy and its disorders. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:1655-1696

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

This 4-marker screen includes alpha-fetoprotein (AFP), estriol (uE3), human chorionic gonadotropin (total beta-hCG: ThCG), and inhibin A. Analyte values are compared to median values at a given gestational age and multiple of the median (MoM) results obtained. The MoM results are used in a multivariate algorithm that includes the mother's age to derive risk factors for trisomy 21 (Down syndrome) and trisomy 18 (Edwards syndrome). The screen for neural tube defects (NTD) uses the AFP MoM only. An interpretive report will be provided. The Beckman Access AFP, ThCG, uE3, and inhibin A assays are automated immunoenzymatic assays with paramagnetic separation and chemiluminescent detection.(Package insert: Beckman Coulter Access AFP, Total bhCG, Unconjugated Estriol, and Inhibin A. Beckman Coulter; 2019)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

4 to 6 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

9 months

Performing Laboratory Location
Indicates the location of the laboratory that performs the test


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Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.


LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
QUAD1 QUAD SCRN (2nd Tri) Maternal, S 48800-7
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
IDD Insulin dependent diabetes 44877-9
IVFP IVF pregnancy 47224-1
MULTF Number of Fetuses 55281-0
10334 Down syndrome screen risk estimate 43995-0
10335 Down syndrome maternal age risk 49090-4
10337 Trisomy 18 screen risk estimate 43994-3
10356 INTERPRETATION 49092-0
10248 Additional comments 48767-8
7058 Recalculated Maternal Serum Screen 32399-8
3009 Specimen collection date 33882-2
7823 Maternal date of birth 21112-8
7834 Calculated age at EDD 43993-5
26717 Maternal Weight 29463-7
26718 Maternal Weight 29463-7
10353 hCG, TOTAL 83086-9
10054 EDD by U/S scan 11781-2
7753 EDD by LMP 11779-6
7203 GA on collection by U/S scan 11888-5
7204 GA on collection by dates 11885-1
7830 GA used in risk estimate 21299-3
10351 AFP 83073-7
10352 uE3 2250-9
10354 INHIBIN 2478-6
113146 Results Summary 32399-8
113147 Neural tube defect risk estimate 48803-1
113148 AFP MoM 23811-3
113149 uE3 MoM 21264-7
113150 hCG, TOTAL MoM 23841-0
113151 INHIBIN MoM 36904-1
RACE1 Patient race 21484-1
SMKNG Current cigarette smoking status 64234-8
CHOR_ Number of Chorions 92568-5
PRHIS Prev Down (T21) / Trisomy Pregnancy 53826-4
PRNTD Prev Pregnancy w/ Neural Tube Defect 53827-2
PTNTD Patient or father of baby has a NTD 53827-2
INTL Initial or repeat testing 77202-0
DRPHN Physician Phone Number 68340-9
601921 AFP MoM (14,0-14,6) 23811-3

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports

Test Update Resources

Change Type Effective Date
Test Changes - Specimen Information 2021-12-14