Test Catalog

Test ID: CORT    
Cortisol, Serum

Specimen Type Describes the specimen type validated for testing


Ordering Guidance

The preferred screening test for Cushing syndrome measures 24-hour urinary free cortisol. Order CORTU / Cortisol, Free, 24 Hour, Urine.


For confirming the presence of synthetic steroids, order SGSS / Synthetic Glucocorticoid Screen, Serum.


For patients taking exogenous glucocorticoids, order CORTU / Cortisol, Free, 24 Hour, Urine.


For evaluating response to metyrapone, order DCORT / 11-Deoxycortisol, Serum.


For evaluation of congenital adrenal hyperplasia, the following tests provide better, accurate, and specific determination of the enzyme deficiency:

-DCORT / 11-Deoxycortisol, Serum

-OHPG / 17-Hydroxyprogesterone, Serum

-DHEA_ / Dehydroepiandrosterone (DHEA), Serum

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions:

1. Morning (8 a.m.) and afternoon (4 p.m.) specimens are preferred.

2. Serum gel tubes should be centrifuged within 2 hours of collection.

3. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.

Additional Information:

1. Include time of collection.

2. If multiple specimens are collected, send separate order for each specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia OK

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
SerumRefrigerated (preferred)7 days
 Frozen 7 days