Test Catalog

Test ID: PMSBB    
Postmortem Screening, Bile and Blood Spot

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Postmortem Screening Algorithm in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Whole blood

Necessary Information

Request the original newborn screening card from the state laboratory where the decedent was born. See Request for Original Newborn Screening Card in Special Instructions.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Both bile and blood spots are required.


Supplies: Card-Postmortem Screening (Filter Paper) (T525)

Collection Container/Tube: Postmortem Screening Card

Specimen Volume: Properly completed screening card

Collection Instructions:

1. Collect blood in a heparin-containing tube and drop 25 mcL of blood onto the 2 circles labeled Blood.

2. Collect bile by direct puncture of the gallbladder and drop 25 mcL of bile onto the 2 circles labeled Bile.

3. Allow to dry at ambient temperature in a horizontal position for 3 or more hours.

4. Fill out information on page 2 of collection card.

5. Do not expose specimen to heat or direct sunlight.

6. Do not stack wet specimens.

7. Keep specimen dry.

Special Instructions Library of PDFs including pertinent information and forms related to the test


If not ordering electronically, complete, print, and send an Inborn Errors of Metabolism Test Request (T798) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Bile spot: 1
Blood spot: 1

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Whole bloodAmbient (preferred)FILTER PAPER
 Refrigerated FILTER PAPER