TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: ANPAT    
Anatomic Pathology Consultation, Wet Tissue

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

An interpretation will be provided by Mayo staff pathologists within a formal pathology report that is sent to the referring pathologist.

 

This test should be used when submitting lung tissue for immunofluorescence, cardiac biopsies, enucleated eye specimens, iris, conjunctiva, cornea and other small eye biopsies for Mayo pathology consultation/interpretation.

 

Appropriate stains and other ancillary testing may be performed and charged separately.

 

This test is not intended for suspected hematologic disorders.

 

Note: Tests requested by the referring physician (eg, immunostains, molecular studies, etc.) may not be performed if they are deemed to be unnecessary by the reviewing Mayo Clinic pathologist. For all pathology consultations, ancillary testing is ordered at the discretion of the Mayo pathologist.

 

See Pathology Consultation Ordering Algorithm in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Varies

Advisory Information

For a pathology consultation on paraffin embedded tissue and slides only (ie, no wet tissue being sent to be processed), order PATHC / Pathology Consultation.

Additional Testing Requirements

Lung biopsy specimens: Immunofluorescence on lung tissue must be ordered in conjunction with this test.

 

Vitreous fluid/aqueous humor specimens: Call 800-533-1710 to request an Expedite on this order since vitreous fluid will have no fixative and needs to reach the laboratory ASAP.

Shipping Instructions

Vitreous fluid/aqueous humor specimens: ship overnight Attention: Ophthalmic Pathology Working Group.

 

Attach the green pathology address label to the outside of the transport container.

Necessary Information

1. Collection date and patient date of birth are required. All specimens must be labeled with specimen type.

2. Although a complete pathology report is not expected to be submitted with the specimen, a preliminary pathology report is required in order to ensure that the specimen is known to a referring pathologist. The name and phone number of the ordering pathologist and a brief history are essential to achieve a consultation fully relevant to the ordering physician's needs.

3. The referring pathologist's name and phone number are required.

4. All specimens, patient history, and requests should be clearly labeled with correct patient identifiers and pathology accession/case number.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Specimen Type: Lung biopsy

Supplies:

-Pathology Packaging Kit (T554)

-Formalin, 10% (T118)

-Michel's Transport Media for Immunofluorescent Testing on Tissue (T321)

Specimen Volume: Entire specimen

Collection Instructions:

1. Submit portion of lung tissue in 10% neutral buffered formalin for light microscopy processing. Alternatively, submit a representative hematoxylin and eosin (H and E) slide from the light microscopy sample along with the tissue in Michel's transport media. Pathology Packaging Kit can be used to submit H and E slide.

2. Place a portion of the unfixed lung tissue in Michel's transport media for immunofluorescence.

 

Specimen Type: Cardiac biopsy

Supplies:

-Pathology Packaging Kit (T554)

-Formalin, 10% (T118)

-Michel's Transport Media for Immunofluorescent Testing on Tissue (T321)

-Electron Microscopy Kit (T660)

-Gluta (Trumps) (T130)

Specimen Volume: Entire specimen

Collection Instructions:

1. For ideal analysis, collect 4 to 6 biopsy specimens and submit all in 10% neutral buffered formalin unless electron microscopy (EM) is requested.

2. If EM is needed, submit at least 1 biopsy into 2.5% to 3% buffered glutaraldehyde preservative using the Electron Microscopy Kit along with the formalin biopsy specimens. If formalin biopsy specimens are not available, light microscopic slides would also be acceptable.

Additional Information: If the question is drug toxicity, storage disease, or if the patient is younger than 25 years old, send at least 1 biopsy specimen in 2.5% to 3% buffered glutaraldehyde in case EM is needed for diagnosis.

 

Specimen Type: Cardiac explant

Supplies:

-Pathology Packaging Kit (T554)

-Refrigerate/Ambient Shipping Box, 5 lb (T329)

Specimen Volume: Entire specimen

Collection Instructions:

1. Fix entire explant specimen in formalin for a minimum of 24 hours.

2. Following fixation, lightly wrap specimen in formalin-soaked paper towel, place in a leak-proof sealable bag with a small amount of formalin, seal, and ship ambient in a sturdy shipping container (ie, Refrigerate/Ambient Shipping Box, 5 lb).

Additional Information: Include the last pretransplantation echocardiogram with report and images as well as all other relevant clinical documents.

 

Specimen Type: Enucleated eye

Supplies:

-Pathology Packaging Kit (T554)

-Formalin - 10% Histo Prep, 45 mL (T117)

Specimen Volume: Entire specimen

Collection Instructions:

1. Immediately after removal, place specimen in approximately 300 mL of 10% neutral buffered formalin.

2. Enucleated eye should not be opened or punctured.

3. After 48 hours in the originating laboratory, the specimen should be transferred from the larger, 300-mL container to a smaller container with fixative (Formalin - 10% Histo Prep, 45 mL) to be shipped without risk of leakage.

 

Specimen Type: Iris, conjunctiva, cornea, and other small biopsies from eye

Supplies:

-Pathology Packaging Kit (T554)

-Formalin - 10% Histo Prep, 45 mL (T117)

Specimen Volume: Entire specimen

Collection Instructions:

1. Obtain biopsy.

2. Place iris, conjunctiva, cornea, and other small biopsies on a paper mount (a piece of filter paper or other porous paper). This helps to keep the specimen from curling when it is fixed.

3. Immediately but gently place specimen (on the paper mount) into 10% formalin, approximately 20 times the volume of the biopsy (Formalin - 10% Histo Prep, 45 mL).

4. Placing sutures at the margins of resection with accompanying explanatory draft will help orientation of the specimen in the lab, in cases where margins are important.

 

Specimen Type: Vitreous fluid/Aqueous humor

Supplies:

-Pathology Packaging Kit (T554)

-Aliquot Tube, 5 mL (T465)

Specimen Volume: Entire specimen

Collection Instructions:

1. Obtain liquid specimen.

2. Place the liquid (vitreous or aqueous) concentrate into a small tube with a screw-top cap. Seal the tube tightly.

3. Put the specimen immediately on a refrigerated cool pack (not dry ice or frozen cool pack).

4. If a washing (diluted) is available, it can also be sent in similar manner.

Specimen Stability Information: Refrigerated

Special Instructions Library of PDFs including pertinent information and forms related to the test

Forms

If not ordering electronically, complete, print, and send a Cardiovascular Test Request (T724) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

See Specimen Required

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

No specimen should be rejected.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
VariesAmbient (preferred)
 Frozen 
 Refrigerated