Test Catalog

Test ID: HGBCE    
Hemoglobin Variant, A2 and F Quantitation, Blood

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Benign Hematology Evaluation Comparison in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Whole Blood EDTA

Advisory Information

This test is intended for monitoring purposes, such as the increase in hemoglobin F (Hb F) after therapy, or the levels of hemoglobin variants after transfusion. The HPFH / Hemoglobin F, Red Cell Distribution, Blood test is a flow cytometry assay that determines the distribution of Hb F within red blood cells.


If the patient has never been appropriately studied, hemoglobin electrophoresis is necessary (see HBELC / Hemoglobin Electrophoresis Cascade, Blood).

Necessary Information


Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing


Preferred: Lavender top (EDTA)

Acceptable: ACD, heparin

Specimen Volume: 4 mL

Collection Instructions:

1. Submit fresh specimen.

2. Do not transfer blood to other containers.

Special Instructions Library of PDFs including pertinent information and forms related to the test


1. Thalassemia/Hemoglobinopathy Patient Information (T358) in Special Instructions

2. If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis


Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTime
Whole Blood EDTARefrigerated10 days