Test Catalog

Test ID: PCPDS    
Plasma Cell Proliferative Disorder, FISH, Bone Marrow

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test is designed for diagnostic specimens from patients with multiple myeloma or other plasma cell proliferative disorders. If a request for testing has been submitted within 12 months of a complete and informative plasma cell proliferative disorder fluorescence in situ hybridization (FISH) study, the current test request will be cancelled.


This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.


When this test is ordered, pre-analysis cell sorting will be performed at an additional charge.


For diagnostic samples, all probes in the initial panel will be evaluated if sufficient plasma cells are identified. The initial panel includes testing for the following abnormalities using the probes listed:

17p-, TP53/D17Z1

1q gain, TP73/1q22

14q32 rearrangement, IGH


Based on the results from the initial panel, reflex testing may be performed to identify the following abnormalities using the probes listed:

t(11;14), CCND1/IGH

t(14;16)(q32;q23) IGH/MAF

t(4;14)(p16.3;q32) FGFR3/IGH

t(14;20)(q32;q12) IGH/MAFB 


For follow-up samples, only TP73/1q22, TP53/D17Z1 and MYC probes will be tested. If a diagnostic sample was uninformative for a probe set due to an insufficient number of plasma cells, attempts may be made to achieve results for the missing probe on a subsequent sample (if sufficient plasma cells are identified).


Initial screening will be performed to determine if sufficient plasma cells are present within the provided specimen.


If the specimen is received greater than 96 hours from collection, or arrives clotted or hemolyzed, this test will be canceled and MFCF / Myeloma, FISH, Fixed Cells will be added as the more appropriate test.

Specimen Type Describes the specimen type validated for testing

Bone Marrow

Advisory Information

This assay detects high risk abnormalities plus CCND1/IGH fusion observed in the bone marrow of patients with a plasma cell disorder.

-For a more complete genetic evaluation, order MPCDS / mSMART, Plasma Cell Proliferative Disorder, FISH, Bone Marrow.

-For paraffin-embedded tissue specimens, order PLASF / Plasma Cell Proliferative Disorder, FISH, Tissue.

-For fixed cell pellet specimens, order MFCF / Myeloma, FISH, Fixed Cells.

-Testing will be changed to the appropriate test if this test is ordered on either of the previous specimens or if bone marrow specimens are received more than 96 hours from collection.

Shipping Instructions

1. Specimen should arrive within 96 hours of collection.

2. Advise Express Mail or equivalent if not on courier service.

Necessary Information

1. Provide a reason for testing with each specimen. The laboratory will not reject testing if this information is not provided but appropriate testing and interpretation may be compromised or delayed.

2. A pathology and/or flow cytometry report may be requested to optimize testing and aid in interpretation of results.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing


Preferred: Yellow top (ACD)

Acceptable: Lavender top (EDTA) or green top (heparin)

Specimen Volume: 4 mL

Collection Instructions: Invert several times to mix bone marrow


If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

2 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Bone MarrowAmbient (preferred)4 days
 Refrigerated 4 days