Test Catalog

Test ID: NSFIB    
Nonalcoholic Steatohepatitis (NASH)-FibroTest, Serum and Plasma

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test is a patented test algorithm developed by BioPredictive. It combines 10 standard biomarkers: gamma-glutamyltransferase (GGT), total bilirubin, alpha-2-macroglobulin, apolipoprotein A1, haptoglobin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol, triglycerides, and fasting glucose. These markers are weighted depending on the patient's age and gender.


Testing is compliant with BioPredictive's technical recommendations and approvals.

Specimen Type Describes the specimen type validated for testing

Plasma NaFl-KOx

Necessary Information

Age and sex are required.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Both serum and plasma are required for this test.


Patient Preparation: Fasting for 12 hours or more is required


Specimen Type: Serum

Supplies: Amber Frosted Tube, 5 mL (T192)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Amber vial

Specimen Volume: 4 mL

Collection Instructions:

1. Centrifuge and aliquot serum into an amber vial within 2 hours of collection.

2. Centrifuged serum must be light protected within 4 hours of collection. It is acceptable to draw the blood and then protect it from light after centrifugation as long as it's within 4 hours of collection.

3. Label specimen as serum.


Specimen Type: Plasma

Collection Container/Tube: Grey top (potassium oxalate/sodium fluoride)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge and aliquot plasma into plastic vial.

2. Label specimen as plasma.


If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Client Test Request (T728) with the specimen

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Serum: 2 mL
Plasma: 0.25 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Patients <14 years of age Reject

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Plasma NaFl-KOxRefrigerated (preferred)7 days
 Frozen 14 days
 Ambient 7 days
SerumRefrigerated (preferred)7 daysLIGHT PROTECTED
 Frozen 14 daysLIGHT PROTECTED
 Ambient 24 hoursLIGHT PROTECTED