Test Catalog

Test ID: HBRP    
Histoplasma capsulatum/Blastomyces species, Molecular Detection, PCR, Varies

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Meningitis/Encephalitis Panel Algorithm in Special Instructions.

Specimen Type Describes the specimen type validated for testing


Advisory Information

Urine is not an acceptable source for this assay. Studies indicate that Histoplasma DNA is not routinely found in the urine of patients with disseminated histoplasmosis. Therefore, the UHIST / Histoplasma Antigen, Urine is the recommended test for this specimen source.

Additional Testing Requirements

This test should always be performed in conjunction with fungal culture, order FGEN / Fungal Culture, Routine.

Shipping Instructions

Specimen must arrive within 7 days of collection; specimens received after 7 days will be rejected.


NALC/NaOH-digested specimen must arrive within 7 days of digestion.

Necessary Information

Specimen source is required.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Histoplasma or Blastomyces species DNA is not likely.


Submit only 1 of the following specimens:


Specimen Type: Body fluid

Sources: Body, CSF, bone marrow

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Type: Respiratory

Sources: BAL, bronchial washing, sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Type: Tissue or bone

Container/Tube: Sterile container

Specimen Volume: 5-10 mm

Collection Instructions: Collect a fresh tissue or bone specimen.



Specimen Type: NALC/NaOH-digested respiratory specimens

Sources: Lavage fluid, bronchial washing, gastric washing, respiratory fluid, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions:

1. Submit digested specimen treated with NALC/NaOH.

2. Clearly indicate on container and order form that specimen is a digested specimen.

Special Instructions Library of PDFs including pertinent information and forms related to the test


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Body Fluid or Respiratory Specimen: 0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Other Specimen in anaerobe vial or viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth) Feces Swab Tissue in formalin fluid Urine Specimen >7 days old

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
VariesRefrigerated (preferred)7 days
 Ambient 7 days
 Frozen 7 days