Test Catalog

Test ID: PLASF    
Plasma Cell Proliferative Disorder, FISH, Tissue

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test does not include a pathology consult. If a pathology consult is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge.


This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.Indicate if the entire panel is to be performed. If the patient is being tracked for known abnormalities, indicate which probes should be used.


Panel includes testing for the following abnormalities using the probes listed:

17p-, TP53/D17Z1

1q gain, TP73/1q22

t(14;16)(q32;q23), IGH/MAF

t(4;14)(p16.3;q32), FGFR3/IGH

8q24.1 rearrangement, MYC

-13/13q-, RB1/LAMP1

+9/+15, D9Z1/D15Z4

+3/+7, D3Z1/D7Z1

14q32 rearrangement, IGH

t(11;14), CCND1/IGH

t(14;20)(q32;q12), IGH/MAFB

t(6;14)(p21;q32), CCND3/IGH


If no probes are specified, probes will be selected based on disease risk stratification, pathologic review, and history (if applicable).

Specimen Type Describes the specimen type validated for testing


Advisory Information

-For fresh bone marrow specimens, order PCPDS / Plasma Cell Proliferative Disorder, FISH, Bone Marrow.

-For fixed cell pellet specimens, order MFCF / Myeloma, FISH, Fixed Cells.

-Testing will be changed to the appropriate test if this test is ordered on either of the previous specimens or if bone marrow specimens are received greater than 96 hours from collection.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

A reason for referral and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology, or surgical pathology reports.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:


Specimen Type: Tissue

Preferred: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.


Acceptable: Slides

Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.


If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

See Specimen Required

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
TissueAmbient (preferred)