Test Catalog

Test ID: CULAF    
Culture for Genetic Testing, Amniotic Fluid

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing amniocytes for prenatal genetic testing.

 

Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.

Specimen Type Describes the specimen type validated for testing

Amniotic Fld

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

 

The additional tests desired must be indicated on the request form that accompanies the specimen.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted.

 

Container/Tube: Amniotic fluid container

Supplies: Refrigerate/Ambient Shipping Box, 5 lb (T329)

Specimen Volume: 5 to 10 mL

Collection Instructions:

1. Discard the first 2 mL of amniotic fluid. If the culture will be performed in conjunction with other cytogenetic testing such as CHRAF / Chromosome Analysis, Amniotic Fluid or CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling, a total of 25 to 30 mL will be needed.

2. Place the tubes in a Styrofoam container.

3. Fill remaining space with packing material.

4. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.

5. Bloody specimens are undesirable.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Amniotic FldRefrigerated (preferred)
 Ambient