Test Catalog

Test ID: FBIOT    
Vitamin B7, H (Biotin)

Method Description Describes how the test is performed and provides a method-specific reference

The Biological Assay employed to quantify the level of biotin in sera uses Lactobacillus plantarum as a biotin-dependent microorganism that requires Biotin for growth. The assay consists of a sterile 96-well microplate. The assay is set by placing standard curve volumes, assay media, control serum and test samples in a sterile 96-well plate and adding a set volume of diluted bacterial culture. This culture is placed in last to allow for equal growth and distribution. The bioassay is placed in 37 degrees celsius  (+/- 3 degrees celsius), humidified, 5% (+/-1%) CO2 incubator and allowed to incubate 64 to 86 hours. The plate is read for %Transmission. This method measures the ability of light to pass through the bacterial culture inversely measuring bacterial growth by biotin utilization.

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.


Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 - 4 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

9 - 17 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test

BioAgilytix Diagnostics