Test Catalog

Test ID: CDG    
Carbohydrate Deficient Transferrin for Congenital Disorders of Glycosylation, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Samples are prepared by diluting serum in water. The sample is injected into an immunoaffinity column and washed. Following 2 wash steps, proteins are then eluted and introduced into an API 4000 tandem mass spectrometer equipped with a Turbo V source configured for electrospray ionization. The mass spectrometer is operated in positive Q1 scan mode with 2 scan ranges; m/z 1090-2000 for apolipoprotein CIII and m/z 2000-3000 for transferrin. Relative quantitation of carbohydrate deficient transferrin and apolipoprotein CIII is achieved by comparing glycoform ratios in each protein.(Lacey JM, Bergen R, Magera MJ, et al: Rapid determination of transferrin isoforms by immunoaffinity liquid chromatography and electrospray mass spectrometry. Clin Chem 2001;47:513-518; Hipgrave Ederveen AL, de Haan N, Baerenfaenger M, Lefeber DJ, Wuhrer M: Dissecting total plasma and protein-specific glycosylation profiles in congenital disorders of glycosylation. Int J Mol Sci. 2020 Oct 15;21(20):7635. doi: 10.3390/ijms21207635)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday, Thursday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

5 to 10 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 month

Performing Laboratory Location Indicates the location of the laboratory that performs the test