Test Catalog

Test ID: ROTA    
Rotavirus Antigen, Feces

Method Description Describes how the test is performed and provides a method-specific reference

This FDA-approved kit utilizes monoclonal antibodies in a solid-phase sandwich type EIA. Plastic microtiter wells are coated with a monoclonal antibody directed against the product of the sixth viral gene (VP6), which is the group-specific antigen for all known human rotaviruses. An aliquot of fecal suspension is added to the well and incubated simultaneously with an anti-rotavirus monoclonal antibody conjugated to horseradish peroxidase, resulting in the rotavirus antigen being sandwiched between the solid-phase and enzyme-linked antibodies. After 60 minutes incubation at ambient temperature, the specimen well is washed in order to remove unbound enzyme-labeled antibodies. Enzyme substrate (urea peroxidase) and chromogen tetra methylbenzidine (TMB) are added to the wells and incubated for 10 minutes at ambient temperature. The enzyme bound in the wells converts the colorless substrate to a blue color. The intensity of the blue color is directly proportional to the concentration of rotavirus antigen in the specimen.(Package insert: Premier Rotaclone EIA for detection of Rotavirus Antigen in Human Fecal Samples. January 2015)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Saturday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Until reported

Performing Laboratory Location Indicates the location of the laboratory that performs the test