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Coenzyme Q10 (CoQ10) is extracted from plasma with cold 1-propanol containing coenzyme Q9 as an internal standard. An aliquot of the lipid extract is fractionated by high-performance liquid chromatography (HPLC). The separated coenzyme Q10 reduced and coenzyme Q10 oxidized are quantified by measurement of selective coulometric response emerging from the chromatographic column.(Tang PH, Miles MV, DeGrauw A, et al: HPLC analysis of reduced and oxidized coenzyme Q[10] in human plasma. Clin Chem 2001 February;47[2]:256-265)
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