Test Catalog

Test ID: FGEN    
Fungal Culture, Routine

Method Description Describes how the test is performed and provides a method-specific reference

Specimens are cultured on selective fungal media (eg,inhibitory mold agar and brain heart infusion blood agar with chloramphenicol and gentamicin). Respiratory sources also are cultured on brain heart infusion agar with chloramphenicol, gentamicin, and cycloheximide. Cultures are incubated for 24 days at 30 degrees C.


Identification of fungi is based on colonial and microscopic morphology, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS), laboratory-developed real-time PCR assays and/or D2 ribosomal RNA (rRNA) gene sequencing, as applicable.(Babady NE, Buckwalter SP, Hall L: Detection of Blastomyces dermatitidis and Histoplasma capsulatum from culture isolates and clinical specimens by use of real-time PCR. J Clin Microbiol 2011;49:3204-3208; Binnicker MJ, Buckwalter SP, Eisberner JJ: Detection of Coccidioides species in clinical specimens by real-time PCR. J Clin Microbiol 2007;45:173-178; Dhiman N, Hall L, Wohlfiel SL: Performance and cost analysis of matrix-assisted laser desorption ionization time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol 2011;49:1614-1616; Hall L, Wohlfiel SL, Roberts GD: Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol 2004;42:622-626; Theel ES, Schmidt BH, Hall L: Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2012;50:3093-3095; Theel ES, Hall L Mandrekar J: Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2011;49:4067-4071)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

24 to 35 days/Positive cultures reported when detected. Preliminary negative report generated at 7 and 14 days.

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Raw specimen saved 7 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test