Test Catalog

Test ID: ESTF    
Estrogens, Estrone (E1) and Estradiol (E2), Fractionated, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Estrogens are fractionated into estradiol and estrone by the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. The LC-MS/MS method employs an organic extraction to remove water-soluble conjugates and to allow for concentration of the specimen. The method is free from interference and represents a reference methodology.


17 Beta-estradiol and estrone are extracted from 0.5 mL of serum with the organic solvent methylene chloride. Deuterated 17 beta-estradiol-d5 and estrone-d4 are added to each specimen before the liquid extraction and serve as internal standards. After derivatization with dansyl chloride, HPLC is used prior to introduction of the derivatized sample extract into the MS/MS.(Bidlingmaier F, Wagner-Barnack M, Butenandt O, Knorr D: Plasma estrogens in childhood and puberty under physiologic and pathologic conditions. Pediatr Res. 1973;7(11):901-907)


The calibration utilizes an 8-point standard curve over a concentration range of 0 to 600 pg/mL.(Anari MR, Bakhtiar R, Zhu B, et al: Derivatization of ethynylestradiol with dansyl chloride to enhance electrospray ionization: application in trace analysis of ethynylestradiol in Rhesus monkey plasma. Anal Chem. 2002;74,4136-4144)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Saturday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

2 to 4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

2 weeks

Performing Laboratory Location Indicates the location of the laboratory that performs the test