Test Catalog

Test ID: LPS    
Lipase, Serum

Method Description Describes how the test is performed and provides a method-specific reference

The lipase method is an enzymatic colorimetric method in which lipase catalyzes the hydrolysis of a natural 1,2-diglyceride to form monoglyceride and fatty acid. Monoglyceride is hydrolyzed by monoglyceride lipase to form glycerol and fatty acid. Glycerol is then phosphorylated by glycerol kinase in the presence of ATP to form glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase to form dihydroxyacetone phosphate and hydrogen peroxide. Subsequently, hydrogen peroxide reacts with 4-aminoamtipyrine and sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine in the presence of peroxidase to form quinone diimine dye. The dye absorbs light at 550 nm. The rate of increase in absorbance at 550 nm is directly proportional to the pancreatic lipase activity in the specimen. The method is sensitive and specific for pancreatic lipase and utilizes co-lipase and deoxycholate as activators.(Package insert: Equal Diagnostics Lipase reagent, Exton, PA. V2.0 03/2019)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location Indicates the location of the laboratory that performs the test