Test Catalog

Test ID: ALPS    
Alpha Beta Double-Negative T Cells for Autoimmune Lymphoproliferative Syndrome, Blood

Method Description Describes how the test is performed and provides a method-specific reference

This assay uses a 5-color, single-platform method with a 2-tube panel stained for the following antibodies: CD3, CD4, CD8, CD45, alpha beta TCR, and B220. The sample is stained with the antibody cocktail and incubated in the dark at room temperature for 20 minutes. Following incubation, the samples are treated with BD lysing solution to lyse the RBCs, followed with a wash step using BD FACS wash buffer. The cells are resuspended in 1% paraformaldehyde and analyzed by flow cytometry. The different subsets are expressed as a percent of CD3 T cells, and the absolute counts of all subsets are expressed as cells/mcL.(Unpublished Mayo method; Bleesing JJH, Brown MR, Dale JK, et al: TCR alpha beta + CD4-CD8- T-cells in humans with the autoimmune lymphoproliferative syndrome express a novel CD45 isoform that is analogous to murine B220 and represents a marker of altered O-glycan biosynthesis. Clin Immunol 2001;100[3]:314-324)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

3 to 4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

4 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test