Test Catalog

Test ID: HBA1C    
Hemoglobin A1c, Blood

Method Description Describes how the test is performed and provides a method-specific reference

The D-100 hemoglobin A1c (HbA1c) test utilizes principles of ion-exchange high-performance liquid chromatography. The samples are automatically diluted on the D-100 and injected into the analytical cartridge. The D-100 delivers a programmed buffer gradient of increasing ionic strength to the cartridge, where the hemoglobins are separated based on their ionic interactions with the cartridge material. The separated hemoglobins then pass through the flowcell, where changes in the absorbance at 415 nm are measured. The D-100 software collects raw data from each analysis and calculates HbA1c values based on a bilevel calibration curve. The HbA1c area is calculated using an exponentially modified Gaussian algorithm. A sample report and a chromatogram are generated for each sample.(Instruction manual: Bio-Rad D-100 HbA1c Instructions For Use, LB0002870revA; 2014)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday; Continuously

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

1 day

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test