Web: | mayocliniclabs.com |
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Email: | mcl@mayo.edu |
Telephone: | 800-533-1710 |
International: | +1 855-379-3115 |
Values are valid only on day of printing. |
A 3-mm disk is punched out of the blood spot onto a 96-well plate. The amino acids are extracted by the addition of methanol and known concentrations of isotopically labeled amino acids as internal standards. The extract is moved to another 96-well plate and dried under a stream of nitrogen. Analytes are measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). The concentrations of the phenylalanine and tyrosine are established by computerized comparison of ion intensities of these analytes to that of the respective internal standards.(Unpublished Mayo method)
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