Test Catalog

Test ID: PLAFL    
Platelet Surface Glycoprotein by Flow Cytometry, Blood

Method Description Describes how the test is performed and provides a method-specific reference

Flow cytometric immunophenotyping of peripheral blood platelets is performed using the following antibodies:


Panel: CD41 (IIb), CD42a (IX), CD42b (Ib-alpha), CD49b (GPIa), CD61 (GPIIIa), and GPVI.

For sample quality purposes CD62P is evaluated.


Using whole blood collected in ACD (-A or -B), platelet surface GPIa, Ib-alpha, IIb, IIIa, VI and IX expression levels are measured by flow cytometry method. Platelets in whole blood are stained with various fluorochrome-labeled primary antibodies and fixed. Then the platelet surface fluorescent intensities of various bound antibodies are measured by flow cytometers. Platelets are first gated by forward and side scatter. Mean fluorescent intensities are recorded and converted to percentage of a median fluorescent intensity of a normal donor study of 20 healthy donors. If the percentage of expression of a glycoprotein (GP) is lower than the corresponding normal range, a deficiency of a GP is detected.(Unpublished Mayo Method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Specimens are processed Monday through Sunday.

Results reported Monday through Friday.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test