TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: HCVL    
Hepatitis C Virus Antibody Confirmation, Serum

Method Description Describes how the test is performed and provides a method-specific reference

The MP Diagnostics Hepatitis C Virus (HCV) Blot 3.0 is a line immunoassay that utilizes a nitrocellulose strip containing 4 bands corresponding to recombinant HCV proteins derived from the capsid (C), NS3, NS4, and NS5 regions of the HCV genome. The HCV proteins are expressed as glutathione-S-transferase (GST) fusion proteins, so a GST control band is also present on each strip to indicate reactivity to native GST. Each strip also contains an IgG control band and an anti-IgG band. Individual strips are incubated with either diluted clinical serum specimens or assay controls. Specific antibodies to HCV, if present in the specimen, will bind to the HCV proteins on the strips. The strips are washed to remove unbound materials and then incubated with purified antihuman IgG conjugated with alkaline phosphatase. The conjugated antibody will bind to any antigen-antibody complexes formed on the strips. Unbound conjugate is removed by washing. A BCIP/NBT (5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium) substrate is added to visualize reactive protein bands on the strips.(Package insert: MP Diagnostics HCV Blot 3.0. MP Biomedicals Asia Pacific Pte Ltd, Publ. MAD 0012-ENG-2, 10/2010)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Wednesday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

14 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester