Test Catalog

Test ID: HEVQU    
Hepatitis E Virus RNA Detection and Quantification, Real-Time RT-PCR, Serum

Method Description Describes how the test is performed and provides a method-specific reference

This assay utilizes real-time polymerase chain reaction (PCR) technology for the qualitative and quantitative detection of hepatitis E virus (HEV) RNA in human serum. Testing involves 3 major processes with the ELITe InGenius integrated system: 1) automated extraction and purification of viral RNA; 2) reverse transcription of viral target RNA sequence to generate complementary DNA (cDNA); and 3) PCR amplification of a 69-base pair sequence in the 5’ end of the HEV open reading frame (ORF) 2 that encodes the viral capsid protein, followed by real-time detection of fluorescent dye-labeled oligonucleotide probes that allow the specific and simultaneous detection and quantification of the target sequence as well as an MS2 internal control (IC) The assay is calibrated to the First World Health Organization (WHO) International Standard for HEV RNA, PEI code 6329/10.(Germer JJ, Ankoudinova I, Belousov YS, et al: Hepatitis E virus detection and quantification by an RT-PCR assay calibrated to the World Health Organization Standard for HEV RNA.  J Clin Microbiol. 2017;55:1478-1487. doi: 10.1128/JCM.02334-16)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.


Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 10 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

2 months

Performing Laboratory Location Indicates the location of the laboratory that performs the test