Test Catalog

Test ID: P53CA    
Hematologic Neoplasms, TP53 Somatic Mutation, DNA Sequencing Exons 4-9, Varies

Method Description Describes how the test is performed and provides a method-specific reference

Peripheral blood specimens from chronic lymphocytic leukemia (CLL) patients only will be analyzed by a screening flow cytometry method to determine B-cell content and confirm the presence of a clonal B-cell population. Blood samples (but not bone marrows) from patients with CLL are enriched for B-lymphocytes by cell sorting and DNA is extracted from the B-cell fraction. For other sample types (bone marrow, fresh or frozen tissues) DNA is extracted directly without prior enrichment. Polymerase chain reaction (PCR) and Sanger sequencing of TP53 exons 4 to 9 is performed. Sequence analysis is performed using Mutation Surveyor and Alamut software. The presence of a detected variant is then assessed using curated public databases of known TP53 gene mutations.(The TP53 Web Site entry UMD TP53 Mutation Database. Accessed 12/3/2013; den Dunnen JT, Antonarakis SE: Mutation nomenclature extensions and suggestions to describe complex mutations: a discussion. Hum Mutat. 2000;15:7-12)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

7 to 14 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

DNA 3 months

Performing Laboratory Location Indicates the location of the laboratory that performs the test