Test Catalog

Test ID: HGEMP    
Hydroxyglutaric Acids, Glutaric Acid, Ethylmalonic Acid, and Methylsuccinic Acid, Plasma

Method Description Describes how the test is performed and provides a method-specific reference

An aqueous internal standard is added to the plasma specimen. The supernatant is evaporated under heated nitrogen and the residue is then reconstituted prior to injection onto a liquid chromatography-tandem mass spectrometry (LC-MS/MS). The MS/MS is operated in the multiple reaction monitoring (MRM) negative mode to follow the precursor to product species transitions. Separation of the structural isomers 2-hydroxyglutaric acid (2OH-GA) and 3-hydroxyglutaric acid (3OH-GA) as well as glutaric acid (GA), ethylmalonic acid (EMA), and methylsuccinic acid (MSA) is accomplished by the optimization of the LC separation. The ratios of the extracted peak areas of GA, EMA, and MSA to their respective internal standards as determined by LC-MS/MS are used to calculate the concentration of each analyte in the sample.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday, Wednesday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

2 to 7 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

2 months

Performing Laboratory Location Indicates the location of the laboratory that performs the test