Test Catalog

Test ID: TRCHG    
ToRCH Profile IgG, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Detection of IgG-class antibodies to Toxoplasma gondii, rubella, cytomegalovirus, and herpes simplex virus types 1 and 2 is performed on the BioPlex 2200 system, which uses multiplex flow immunoassay technology. Five different populations of fluorescent dyed beads are each coated with antigens unique to each infectious organism. Patient sample is combined with sample diluent and the 5 bead set and incubated at 37 degrees C. During this time IgG antibodies will bind to the antigen-coated beads. After a wash cycle, a fluorescently labeled antihuman IgG antibody conjugate is added to the mixture and incubated at 37 degrees C. Following a wash step to remove unbound conjugate, the bead mixture is passed through a detector that identifies the bead based on dye fluorescence and determines the amount of antibody captured by the antigen based on fluorescence of the antihuman IgG conjugate. Raw data are calculated in relative fluorescence intensity and is converted to an antibody index (AI) for interpretation. Three additional dyed beads, an internal standard bead, a serum verification bead, and a reagent black bead are present in each reaction mixture to verify detector response, the addition of serum to the reaction vessel and the absence of significant nonspecific binding in serum, respectively.(Package inserts: BioPlex 2200 System, ToRC IgG. Bio-Rad Laboratories; 03/2012; BioPlex 2200 System, MMRV IgG. 11/30/2018)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Saturday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test