Test Catalog

Test ID: TYRSC    
Tyrosinemia Follow-Up Panel, Self-Collect, Blood Spot

Method Description Describes how the test is performed and provides a method-specific reference

A 3-mm disk is punched out of the blood spot onto a 96-well plate. The amino acids and nitisinone are extracted by the addition of methanol and known concentrations of isotopically labeled amino acids and mesotrione as internal standards. The extract is moved to another 96-well plate and dried under a stream of nitrogen. In a parallel process, succinylacetone is extracted from the residual blood spot by the addition of a methanol solution containing isotopically labeled succinylacetone as internal standard, derivatized with an acidic hydrazine solution, evaporated and combined with the amino acid and nitisinone extract. Analytes are measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The concentrations of the analytes are established by computerized comparison of ion intensities of these analytes to that of the respective internal standards.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

3 to 5 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 year

Performing Laboratory Location Indicates the location of the laboratory that performs the test