Test Catalog

Test ID: DOCK8    
Dedicator of Cytokinesis 8 (DOCK8) Deficiency, Blood

Method Description Describes how the test is performed and provides a method-specific reference

The dedicator of cytokinesis 8 (DOCK8) protein expression assay is performed on whole blood. Samples are fixed, permeabilized and stained with antibodies specific for CD45, CD14, CD19, CD3, and CD56 along with either the DOCK8 antibody (unconjugated) or isotype control (unconjugated). A secondary mouse anti-rabbit reporter antibody is added to allow the assessment of DOCK8 and isotype control expression. Samples are then analyzed on a flow cytometer. DOCK8 expression is evaluated on the following populations: T-cells: (CD45+CD14[neg]CD3+), B-cells: (CD45+CD14[neg]CD3[neg]CD19+), NK-cells (CD45+CD14[neg]CD3[neg]CD56+), Monocytes (CD45+CD14+).(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday

Specimen must be received by 4p.m. on Friday.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

4 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test