Test Catalog

Test ID: HPCRP    
Helicobacter pylori with Clarithromycin Resistance Prediction, Molecular Detection, PCR, Varies

Method Description Describes how the test is performed and provides a method-specific reference

Viable and nonviable clinical isolates are processed by transferring up to a 1 mcL loop full of isolate into a swab neutralization buffer (NB) tube for thermal/physical lysis and then diluted 1:100 prior to testing. The PCR assay employs a target-specific detection system including primers, as well TaqMan detection probes alongside a SimpleProbe for melt curve analysis-based genotyping targeting the 23S ribosomal RNA gene. The LightCycler 480 II instrument amplifies and monitors target nucleic acid sequences by fluorescence during PCR cycling. Detection of amplified product is based on the TaqMan probe principle. For PCR product detection, the TaqMan probe binds the complementary strand of amplified target. Specific PCR Taq polymerase with 5’-3’ exonuclease activity degrades the probe, releasing the fluorophore and breaking its proximity to the quencher molecule, allowing fluorescence of the fluorophores.. At the conclusion of PCR cycling, amplified product is thermally denatured and then cooled to allow for a fluorescein labeled SimpleProbe to anneal to an 18-base pair region of the amplified target that includes the 2 position mutations associated clarithromycin resistance. The temperature is slowly raised while consistently monitoring fluorescence. The process is completed in a closed system to mitigate contamination. Further, contamination control is achieved through UNG enzymatic treatment and a master mix which includes dUTPs.(Chen D, Cunningham SA, Cole N, et al: Phenotypic and Molecular Antimicrobial Susceptibility of Helicobacter pylori in the United States. Antimicrob Agents Chemother 2016;61:e02530-16)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

3 to 4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

30 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test