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Values are valid only on day of printing. |
The liquid Roche amylase method is an enzymatic colorimetric test using 4,6-ethyliden (G7)-p-nitrophenol (G1)-alpha, D-maltoheptaoside (ethylidene-G7PNP) as a substrate. Human salivary and pancreatic amylases convert the substrate at approximately the same rate. The alpha-amylase cleaves the substrate into G2, G3, G4 PNP fragments. The G2 and G3 and G4 PNP fragments are further hydrolyzed by an alpha-glucosidase to yield p-nitrophenol and glucose. The rate of increase in absorbance at 415 nm (measuring the increase in p-nitrophenol) is proportional to amylase activity.(Package insert: Roche AMYL reagent. Roche Diagnostic Corp; V11.0 12/2019)
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