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Genomic DNA from whole blood or cord blood is amplified by PCR with primers specific for the F8 intron 1 inversion mutation.(Bagnall RD, Waseem N, Green PM, Giannelli F: Recurrent inversion breaking intron 1 of the factor VIII gene is a frequent cause of severe hemophilia A. Blood 2002;99(1):168-174; Meijer P, Verbruggen, Spannagi M: Clotting factors and inhibitors: Assays and Interpretation. Chapter 33. In Laboratory Hematology Practice. Edited by K Kottke-Marchant. Wiley Blackwell Publishing. 2012, pp 435-446)
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